Glutaredoxin-2, 20-164aa, Human, His tag, E.coli

Glutaredoxin-2, as known as GRX2, multifunctional enzyme with glutathione-dependent oxidoreductase, glutathione peroxidase and glutathione S-transferase(GST) activity. This protein is localized both in the mitochondria and nucleus. It has anti-apoptotic activity by preventing cardiolipin oxidation and cytochrome c release. GRX2 exhibited 36% identity with GRX1 and had a disulfide active center containing the Cys-Ser-Tyr-Cys motif. Recombinant human Glutaredoxin 2, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.

Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.

0.5 mg/ml (determined by Bradford assay)

• Elliott SJ., et al. (2009) Biochemistry. 948(18):3813-5.
• Das DK., et al. (2008) J Mol Cell Cardiol. 44(2):252-60

>, 90% by SDS - PAGE

MSAGWLDRAA GAAGAAAAAA SGMESNTSSS LENLATAPVN QIQETISDNC VVIFSKTSCS YCTMAKKLFH DMNVNYKVVE LDLLEYGNQF QDALYKMTGE RTVPRIFVNG TFIGGATDTH RLHKEGKLLP LVHQCYLKKS KRKEFQLEHH HHHH