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Golgi Complex(371-4), CF647 conjugate, 0.1mg/mL

ArtNr B-BNC470102-500
Hersteller Biotium
Menge 500 uL
Quantity options 100 uL 500 uL
Kategorie
Typ Antibody Primary
Format Liquid
Applikationen IF, IHC
Clon 371-4
Specific against Human (Homo sapiens)
Host Mouse
Isotype IgG1 Kappa
Konjugat/Tag CF647
Citations

  1. Cancer Res (2001) 61: 3339-3347. (IF)
  2. J Virol (2005) 79(13): 8506. (IF)

ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias Golgi Complex; Golgi apparatus; Golgi zone
Versandbedingung Raumtemperatur
Lieferbar
Manufacturer - Type
Primary
Manufacturer - Applications
IF (published)|IHC, FFPE (verified)
Manufacturer - Category
Antibodies
Manufacturer - Targets
Golgi Complex
Manufacturer - Conjugate / Tag
CF647
Manufacturer - Host
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Shipping Temperature
Room temperature
Storage Conditions
Store at 2 to 8°C|Protect fluorescent conjugates from light
Manufacturer - Research Area
Organelle markers
Description
This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin
Animal
Ingredient of biological origin
Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Special handling for PI and label
Protect from light
Undated stability guarantee in PI
2 years
Stability at RT during shipping (protected from light)
Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity
41116161
UNSPSC Commodity Title
Primary and secondary antibodies for multiple methodology
immunostaining detection application
Classified or regulated chemicals
0.05% sodium azide (CAS 26628-22-8)
Concentration
0.1 mg/mL
Storage buffer
PBS, 0.1% BSA, 0.05% azide
Dated shelf life printed on label
Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)
SU-DHL-1 large cell lymphoma cells.
Clonality
Monoclonal
Regulatory Status
For research use only (RUO)
Positive Control
HepG2, A431 or HeLa cells. Placenta, Tonsil, Testis and Ovary.
Antibody target cellular localization
Golgi apparatus
Antibody application notes
Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Does not react with mouse or rat, others not known|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes|Immunocytochemistry Acetone or paraformaldehyde fixed 0.5-1 ug/mL|Western blotting 0.5-1.0 ug/mL|Flow Cytometry 0.5-1.0 ug/million cells in 0.1 mL|Optimal dilution for a specific application should be determined by user
Expected Antibody Applications
IF (published for clone)
Manufacturer - Antibody Reactivity
Golgi Complex

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 500 uL
Lieferbar: In stock
lieferbar

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