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Golgi Complex(371-4), CF647 conjugate, 0.1mg/mL

Golgi Complex(371-4), CF647 conjugate, 0.1mg/mL

Item no.	B-BNC470102-500
Manufacturer	Biotium
Amount	500 uL
Quantity options	100 uL 500 uL
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	IF, IHC
Clone	371-4
Specific against	Human (Homo sapiens)
Host	Mouse
Isotype	IgG1 Kappa
Conjugate/Tag	CF647
Citations	Cancer Res (2001) 61: 3339-3347. (IF) J Virol (2005) 79(13): 8506. (IF)
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Golgi Complex; Golgi apparatus; Golgi zone
Shipping Condition	Room temperature
Available
Manufacturer - Type	Primary
Manufacturer - Applications	IF (published)\|IHC, FFPE (verified)
Manufacturer - Category	Antibodies
Manufacturer - Targets	Golgi Complex
Manufacturer - Conjugate / Tag	CF647
Manufacturer - Host	Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Shipping Temperature	Room temperature
Storage Conditions	Store at 2 to 8°C\|Protect fluorescent conjugates from light
Manufacturer - Research Area	Organelle markers
Description	This MAb recognizes an antigen associated with the Golgi complex in human cells only. It can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Product origin	Animal
Ingredient of biological origin	Mus musculus (mouse), BSA from bovine serum (Bos taurus) or recombinant BSA produced in Chinese hamster ovary cells.
Special handling for PI and label	Protect from light
Undated stability guarantee in PI	2 years
Stability at RT during shipping (protected from light)	Stable at room temperature or 37°C (98°F) for 7 days.
UNSPSC Commodity	41116161
UNSPSC Commodity Title	Primary and secondary antibodies for multiple methodology immunostaining detection application
Classified or regulated chemicals	0.05% sodium azide (CAS 26628-22-8)
Concentration	0.1 mg/mL
Storage buffer	PBS, 0.1% BSA, 0.05% azide
Dated shelf life printed on label	Guaranteed for at least 24 months from date of receipt when stored as recommended
Immunogen (Secondaries, for anti tag only)	SU-DHL-1 large cell lymphoma cells.
Clonality	Monoclonal
Regulatory Status	For research use only (RUO)
Positive Control	HepG2, A431 or HeLa cells. Placenta, Tonsil, Testis and Ovary.
Antibody target cellular localization	Golgi apparatus
Antibody application notes	Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody\|Immunofluorescence: 0.5-1 ug/mL\|Does not react with mouse or rat, others not known\|Immunohistology formalin-fixed 0.5-1 ug/mL\|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes\|Immunocytochemistry Acetone or paraformaldehyde fixed 0.5-1 ug/mL\|Western blotting 0.5-1.0 ug/mL\|Flow Cytometry 0.5-1.0 ug/million cells in 0.1 mL\|Optimal dilution for a specific application should be determined by user
Expected Antibody Applications	IF (published for clone)
Manufacturer - Antibody Reactivity	Golgi Complex

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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B-BNC470102-500-datasheet.pdf

B-BNC470102-500-safety-datasheet.pdf