CARD HyperOva

Hersteller Cosmobio
Kategorie Produkte / Mouse in vitro Fertilization
Typ Bio-active substances
Specific against other
Menge 1 ML
ArtNr KYD-010-EX
Eclass 6.1 32160405
Eclass 9.0 32160405
Lieferbar
Composition
1. anti-inhibin antibody

2. Purified equine villus chorionic gonadotropin (eCG)
Protocol
1. Take CARD HyperOva out from the freezer, and thaw it in incubator or water bath at 37C for 10 minutes.

2. Administer the CARD HyperOva to mouse within one hour after thawing. Equalize the CARD HyperOVA by inverting the tube before aspirating it to syringe.

3. You may have some clumps floating* in the solution which you cannot aspirate by the injection needle. In such a case, avoid the clumps and aspirate only other solution.

4. Female mice (C57BL/6 strain, 4 weeks of age) are intraperitoneally administered CARD HyperOva (0.1 - 0.2mL/head; i.p).

5. At 48h after the administration of HyperOva, 7.5IU hCG(i.p.)** are administered to the mice.

6. At 17h after hCG injection, mice are sacrificed by cervical dislocation, and their oviducts are collected and transferred to a fertilization dish.
StoreTmp
-20C
TransTmp
Shipping dry ice
Reference
Takeo T, Nakagata N. Superovulation using the combined administration of inhibin antiserum and equine chorionic gonadotropin increases the number of ovulated oocytes in C57BL/6 female mice. PLoS One. 2015 May 29; 10(5).
Menge: 1 ML
Lieferbar: In stock
Listenpreis: 149,00 €
Rabatt: -5%
Preis: 141,55 €
lieferbar

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Salesmanager: Arne Pelz

Email: sales@hoelzel.de

Arbeitsanleitung

KYD-010-EX.pdf