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Signal-Seeker Phosphotyrosine Enrichment Kit (30 assays)

Signal-Seeker Phosphotyrosine Enrichment Kit (30 assays)

Hersteller	Cytoskeleton
Kategorie	Other Assays & Kits Other Kits
Typ	Assay
Specific against	other
Menge	KIT 30 assays
ArtNr	BK160
eClass 6.1	32161000
eClass 9.0	32161000
Lieferbar
Advantages	Discover and publish novel regulatory mechanisms. Detect highly transient regulation of protein modifications. Use different kits to build a temporal protein regulation profile. Investigate the role of known protein modifications in your system. Obtain data for endogenous proteins. Discover novel biomarkers. Complements and enriches proteomic approaches.
Associated Products	Signal Seeker Ubiquitination Enrichment Kit (Cat. # BK161) Anti-Phosphotyrosine HRP conjugated monoclonal antibody (Cat.# APY03-HRP) Anti-Phosphotyrosine Affinity Beads (Cat.# APY03-beads) Rac Activator I (Cat.# CN02)
Background	Signal Seeker kits use affinity beads to pull-out and enrich modified proteins from any given cell or tissue lysate. The enriched protein population is then analyzed by standard western blot procedures using a primary antibody to the target protein.
Kit contents	The kit contains sufficient reagents to perform 30 phosphotyrosine immunoprecipitation reactions: The following components are included in the kit. BLASTR Lysis Buffer BLASTR Dilution Buffer BLASTR Wash Buffer Protease Inhibitor Cocktail Tyrosine Phosphatase Inhibitor Phosphotyrosine Affinity Beads IP Control Beads Anti-phosphotyrosine-HRP antibody Precision Red Protein Assay Reagent Bead Elution Buffer DMSO Chemiluminescent Reagent A Chemiluminescent Reagent B Spin Columns
Overview	Protein regulation during signal transduction and other cellular events is, by necessity, a rapid and dynamic process. Most often, these mechanisms involve modification of an extremely small fraction of the protein target. This makes the scientists job of capturing key regulatory processes difficult and frustrating. Analysis of key regulatory mechanisms involving phosphorylation, ubiquitination, SUMOylation or acetylation has historically required a huge investment of time and effort.
Shipping Temp.	AT
Storage on Arrival	4C

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