Comparison

Signal-Seeker Phosphotyrosine Enrichment Kit (30 assays)

Manufacturer Cytoskeleton
Category
Type Assay
Specific against other
Amount KIT 30 assays
Item no. BK160
eClass 6.1 32161000
eClass 9.0 32161000
Available
Advantages
Discover and publish novel regulatory mechanisms.
Detect highly transient regulation of protein modifications.
Use different kits to build a temporal protein regulation profile.
Investigate the role of known protein modifications in your system.
Obtain data for endogenous proteins.
Discover novel biomarkers.
Complements and enriches proteomic approaches.
Associated Products
Signal Seeker Ubiquitination Enrichment Kit (Cat. # BK161)
Anti-Phosphotyrosine HRP conjugated monoclonal antibody (Cat.# APY03-HRP)
Anti-Phosphotyrosine Affinity Beads (Cat.# APY03-beads)
Rac Activator I (Cat.# CN02)
Background
Signal Seeker kits use affinity beads to pull-out and enrich modified proteins from any given cell or tissue lysate. The enriched protein population is then analyzed by standard western blot procedures using a primary antibody to the target protein.
Kit contents
The kit contains sufficient reagents to perform 30 phosphotyrosine immunoprecipitation reactions: The following components are included in the kit.
BLASTR Lysis Buffer
BLASTR Dilution Buffer
BLASTR Wash Buffer
Protease Inhibitor Cocktail
Tyrosine Phosphatase Inhibitor
Phosphotyrosine Affinity Beads
IP Control Beads
Anti-phosphotyrosine-HRP antibody
Precision Red Protein Assay Reagent
Bead Elution Buffer
DMSO
Chemiluminescent Reagent A
Chemiluminescent Reagent B
Spin Columns
Overview
Protein regulation during signal transduction and other cellular events is, by necessity, a rapid and dynamic process. Most often, these mechanisms involve modification of an extremely small fraction of the protein target. This makes the scientists job of capturing key regulatory processes difficult and frustrating. Analysis of key regulatory mechanisms involving phosphorylation, ubiquitination, SUMOylation or acetylation has historically required a huge investment of time and effort.
Shipping Temp.
AT
Storage on Arrival
4C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: KIT 30 assays
Available: In stock
available

Delivery expected until 4/18/2024 

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