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Recombinant Escherichia coli Murein hydrolase activator NlpD(nlpD)

ArtNr CSB-EP359913ENV-20
Hersteller Cusabio
Menge 20 ug
Quantity options 1 mg 10 ug 100 ug 20 ug 200 ug 50 ug 500 ug
Kategorie
Typ Proteins Recombinant
Format Liquid or Lyophilized powder
Specific against other
Host E.coli
Konjugat/Tag SUMO, HIS
Purity Greater than 90% as determined by SDS-PAGE.
Sequence CSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKMG TTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQ PVQMENGRIVYNRQYGNIPKGSYSGSTYTVKKGDT LFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQV GNASGTPITGGNAITQADAAEQGVVIKPAQNSTVA VASQPTITYSESSGEQSANKMLPNNKPTATTVTAP VTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIE TFG
Protein Familie E.coli NlpD/Haemophilus LppB family
Citations LytM-domain factors are required for daughter cell separation and rapid ampicillin-induced lysis in Escherichia coli.Uehara T., Dinh T., Bernhardt T.G.J. Bacteriol. 191:5094-5107(2009).
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Lieferbar
Manufacturer - Targets
nlpD
Manufacturer - Conjugate / Tag
N-terminal 6xHis-SUMO-tagged
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Molecular Weight
53.5 kDa
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
General Research Areas
Microbiology
Relevance
Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.
Biologically Active
Not Test
Expression Region
26-379aa
Protein Length
Full Length of Mature Protein
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function
Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.
Subcellular Location
Cell inner membrane, Lipid-anchor

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 20 ug
Lieferbar: In stock
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