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Recombinant Escherichia coli Murein hydrolase activator NlpD(nlpD)

Recombinant Escherichia coli Murein hydrolase activator NlpD(nlpD)

Item no.	CSB-EP359913ENV-20
Manufacturer	Cusabio
Amount	20 ug
Quantity options	1 mg 10 ug 100 ug 20 ug 200 ug 50 ug 500 ug
Category	Food Safety Antibiotics Peptides & Proteins Recombinant Proteins
Type	Proteins Recombinant
Format	Liquid or Lyophilized powder
Specific against	other
Host	E.coli
Conjugate/Tag	SUMO, HIS
Purity	Greater than 90% as determined by SDS-PAGE.
Sequence	CSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKMG TTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQ PVQMENGRIVYNRQYGNIPKGSYSGSTYTVKKGDT LFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQV GNASGTPITGGNAITQADAAEQGVVIKPAQNSTVA VASQPTITYSESSGEQSANKMLPNNKPTATTVTAP VTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIE TFG
Protein Family	E.coli NlpD/Haemophilus LppB family
Citations	LytM-domain factors are required for daughter cell separation and rapid ampicillin-induced lysis in Escherichia coli.Uehara T., Dinh T., Bernhardt T.G.J. Bacteriol. 191:5094-5107(2009).
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Available
Manufacturer - Targets	nlpD
Manufacturer - Conjugate / Tag	N-terminal 6xHis-SUMO-tagged
Storage Conditions	The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20C/-80C. The shelf life of lyophilized form is 12 months at -20C/-80C.
Molecular Weight	53.5 kDa
Buffer	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
General Research Areas	Microbiology
Relevance	Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.
Biologically Active	Not Test
Expression Region	26-379aa
Protein Length	Full Length of Mature Protein
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20C/-80C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Notes	Repeated freezing and thawing is not recommended. Store working aliquots at 4C for up to one week.
Function	Activator of the cell wall hydrolase AmiC. Required for septal murein cleavage and daughter cell separation during cell division.
Subcellular Location	Cell inner membrane, Lipid-anchor

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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CSB-EP359913ENV-20-datasheet.pdf

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Amount: 20 ug

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Delivery expected until 8/28/2025

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