Human Cytokine Array C1 (4)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-CYT-1-4
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio C-Series Human Cytokine Antibody Array 1 Kit. Detects 23 Human Cytokines. Suitable for all liquid sample types.
Features
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
Number of Targets Detected
23
Target Names
GCSF, GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), IL-1 alpha (IL-1 F1), IL-2, IL-3, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL-10, IL-13, IL-15, IFN-gamma, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), MIG (CXCL9), RANTES (CCL5), TGF beta 1, TNF alpha, TNF beta (TNFSF1B)
Targets Detected
CCL2, CCL5, CCL7, CCL8, CXCL1, CXCL8, CXCL9, G-CSF, GM-CSF, GRO, GRO alpha, IFN-gamma, IL-1 alpha, IL-1 F1, IL-10, IL-13, IL-15, IL-2, IL-3, IL-5, IL-6, IL-7, IL-8, MARC, MCP-1, MCP-2, MCP-3, MIG, RANTES, TGF beta 1, TNF alpha, TNF beta, TNFSF1B
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker/ Key Factor Screening, Identifying Key Factors, Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVPs ChemiDoc-It or EpiChem II Benchtop Darkroom), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Membrane
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Chemiluminescence
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
1. Modulation of Cytokine Release and Gene Expression by the Immunosuppressive Domain of gp41 of HIV-1
2. Villar CC, Kashleva H, Mitchell AP, Dongari-Bagtzoglou A. Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection. Infect Immun. 2005, 73:4588-4595.
3. Bohmova K, Hladikova Z, Cerny M , Flajsmanova K, Vrabelova Z, et al. Cord Blood Cytokine Profile Detection in Neonates with T1D Parents–Monitoring of Cellular Auto-reactivity Using Protein Microarray. Scand J Immunol.2007, 66:563–571
4. Joner M, Farb A, Cheng Q, Finn A , Acampado E, et al.Pioglitazone Inhibits In-Stent Restenosis in Atherosclerotic Rabbits by Targeting Transforming Growth Factor- and MCP-1.Arterioscler Thromb Vasc Biol. 2007, 27:182
5. Gruber H E, Hoelscher G, Ingram J A, Chow Y, Loeffler B, et al. 1, 25(OH)2-Vitamin D3 Inhibits Proliferation and Decreases Production of Monocyte Chemoattractant Protein-1, rombopoietin, VEGF, and Angiogenin by Human Annulus Cells In Vitro. Spine. 2008, 33 755-765
6. Liao H, Su S, Chen Y, Chou C, Kuo C.Norcantharidin preferentially induces apoptosis in human leukemic Jurkat cells without affecting viability of normal blood mononuclear cells. Food Chem Toxicol.2007, 45 :1678–1687
7. Pulai JI, Chen H, Im H-J. Kumar S, et al. NF-kappaB Mediates the Stimulation of Cytokine and Chemokine Expression by Human Articular Chondrocytes in Response to Fibronectin Fragments. J Immunol. 2005, 174:5781-5788.
8. Sze S, Kleijn D, Lai R, Tan E, Zhao H, et al.Elucidating the Secretion Proteome of Human Embryonic Stem Cell-derived Mesenchymal Stem Cells.Mol Cell Proteomics. 2007, 6: 1680-1693
9. Patton GW, Powell DA, Hakki A, Friedman H, Pross S. Nicotine modulation of cytokine induction by LPS-stimulated monocytes and coronary artery endothelial cells. Int Immunopharmacol. 2006, 6:26-35.
10. Li L. Oral epithelium–Candida glabrata interactions in vitro.Oral Microbiol Immun.2007: 22: 182–187
11. Turtinen L W , Prall D N , Bremer L A , Nauss R E, Hartsel S C.Antibody Array-Generated Profiles of Cytokine Release from THP-1 Leukemic Monocytes Exposed to Different Amphotericin B Formulations. Antimicrob Agents Chemother. 2004, 48(2):396–403.
12. Yen Y-T, Liao F, Hsiao C-H, Kao C-L, Chen Y-C, Wu-Hsieh BA. Modeling the Early Events of Severe Acute Respiratory Syndrome Coronavirus Infection In Vitro. J Virol. 2006, 80(6):2684-2693.
13. Villar CC, Kashleva H, Mitchell AP, Dongari-Bagtzoglou A. Invasive Phenotype of Candida albicans Affects the Host Proinflammatory Response to Infection. Infect Immun. 2005, 73:4588-4595.
14. Pockaj BA, Basu, GD, Pahtangey LB, Gray RJ, et al. Reduced T-Cell and Dendritic Cell Function Is Related to Cyclooxygenase-2 Overexpression and Prostaglandin E2 Secretion in Patients With Breast Cancer. Annal Surg Oncol. 2004, 11(3):328–339.
15. Sohara Y, Shimada H, Minkin C, Erdreich-Epstein A, Nolta J, et al. Bone Marrow Mesenchymal Stem Cells Provide an Alternate Pathway of Osteoclast Activation and Bone Destruction by Cancer Cells. Cancer Res. 2005, 65(4):1129–1135
16. Norata GD, Tibolla G, Seccomandi PM, Poletti A, Catapano AL. Dihydrotestosterone Decreases Tumor Necrosis Factor-alpha and Lipoploysaccharide-Induced Inflammatory Response in Human Endothelial Cells. J Clin Endocrinol Metab. 2006, 91:546–554.
17. Wang M, Xiao GG, Li N, Xie Y, Loo JA, Nel AE. Use of a fluorescent phosphoprotein dye to characterize oxidative stress-induced signaling pathway components in macrophage and epithelial cultures exposed to diesel exhaust particle chemicals. Electrophoresis. 2005, 26:2092–2108.
18. Choi K-S, Park JT, Dumler JS. Anaplasma phagocytophilum Delay of Neutrophil Apoptosis through the p38 Mitogen-Activated Protein Kinase Signal Pathway. Infect. Immun. December 2005 vol. 73 no. 12 8209-8218
19. Fujiwara, H et al. In vitro induction of myeloid leukemia-specific CD4 and CD8 T cells by CD40 ligand-activated B cells gene modified to express primary granule proteins. In vitro induction of myeloid leukemia-specific CD4 and CD8 T cells by CD40 ligand-activated B cells gene modified to express primary granule proteins. In vitro induction of myeloid leukemia-specific CD4 and CD8 T cells by CD40 ligand-activated B cells gene modified to express primary granule proteins. Clin. Cancer Res. Volume 11, Issue 12, Pages 4495-503, Jun 2005
20. Cattaneo V., Tribulatti M., Carabelli J., et al. Galectin-8 elicits pro-inflammatory activities in the endothelium. Glycobiology, Published Online ahead of print, June 22 2014. doi:10.1093/glycob/cwu060
21. Sos M., et al. Oncogene Mimicry as a Mechanism of Primary Resistance to BRAF Inhibitors. Cell Reports Available online 7 August 2014. DOI: 10.1016/j.celrep.2014.07.010
22. Alkhatib B., et al. ACUTE MECHANICAL INJURY OF THE HUMAN INTERVERTEBRAL DISC: LINK TO DEGENERATION AND PAIN. European Cells and Materials, 2014 (28) 98-111.
23. Nagata E, Fujii N, Hosomichi K, Mitsunaga S, Suzuki Y, et al. (2014) Possible Association between Dysfunction of Vitamin D Binding Protein (GC Globulin) and Migraine Attacks. PLoS ONE 9(8): e105319. doi:10.1371/journal.pone.0105319
24. Lee CH., et al. IL-1beta Promotes Malignant Transformation and Tumor Aggressiveness in Oral Cancer. Journal of Cellular Physiology Sept 2014, Epub Ahead of print. DOI: 10.1002/jcp.24816
25. Nazzal D., et al. Human thymus medullary epithelial cells promote regulatory T-cell generation by stimulating interleukin-2 production via ICOS ligand. Cell Death and Disease (2014) 5, e1420, doi:10.1038/cddis.2014.377

Hinweis: Die dargestellten Informationen und Dokumente (Bedienungsanleitung, Produktdatenblatt und Sicherheitsdatenblatt) entsprechen unserem letzten Update und sollten lediglich der Orientierung dienen. Wir übernehmen keine Garantie für die Aktualität. Für spezifische Anforderungen bitten wir Sie, uns eine Anfrage zu stellen.

Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 650,60 €
Preis: 650,60 €
lieferbar

Vergleichen

Angebot anfordern

Fragen zum Produkt?
 
Schließen