Human Cytokine Array G5 (4)

Hersteller Raybiotech
Typ Array
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-CYT-G5-4
eClass 6.1 32161000
eClass 9.0 32161000
Short description
RayBio G-Series Human Cytokine Antibody Array 5 Kit. Detects 80 Human Cytokines. Suitable for all liquid sample types.
  • High sensitivity and specificity
  • Low sample volume (10-100 ul per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays
Number of Targets Detected
Target Names
ENA-78 (CXCL5), GCSF, GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), I-309 (TCA-3/CCL1), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL-10, IL-12 p40/p70, IL-13, IL-15, IFN-gamma, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), M-CSF, MDC (CCL22), MIG (CXCL9), MIP-1 beta (CCL4), MIP-1 delta (CCL15), RANTES (CCL5), SCF, SDF-1 alpha (CXCL12 alpha), TARC (CCL17), TGF beta 1, TNF alpha, TNF beta (TNFSF1B), EGF, IGF-1, Angiogenin, Oncostatin M, Thrombopoietin (TPO), VEGF-A, PDGF-BB, Leptin, BDNF, BLC (CXCL13), Ck beta 8-1 (CCL23), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), FGF-4, FGF-6, FGF-7 (KGF), FGF-9, Flt-3 Ligand, Fractalkine (CX3CL1), GCP-2 (CXCL6), GDNF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-16, IP-10 (CXCL10), LIF, Light (TNFSF14), MCP-4 (CCL13), MIF, MIP-3 alpha (CCL20), NAP-2 (PPBP/CXCL7), NT-3, NT-4, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PARC (CCL18), PLGF, TGF beta 2, TGF beta 3, TIMP-1, TIMP-2
Targets Detected
Angiogenin, BDNF, BLC, CCL1, CCL11, CCL13, CCL15, CCL17, CCL18, CCL2, CCL20, CCL22, CCL24, CCL26, CCL4, CCL5, CCL7, CCL8, CK beta 8-1, CX3CL1, CXCL1, CXCL10, CXCL12 alpha, CXCL13, CXCL6, CXCL7, CXCL8, CXCL9, EGF, ENA-78, Eotaxin-1, Eotaxin-2, Eotaxin-3, FGF-4, FGF-6, FGF-7, FGF-9, Flt-3 Ligand, Fractalkine, G-CSF, GCP-2, GDNF, GM-CSF, GRO, GRO alpha, HGF, I-309, IFN-gamma, IGF-1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-12 p40/p70, IL-13, IL-15, IL-16, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, KGF, Leptin, LIF, LIGHT, M-CSF, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MIF, MIG, MIP-1 beta, MIP-1 delta, MIP-3 alpha, MPIF-2, NAP-2, NT-3, NT-4, Oncostatin M, Osteopontin, Osteoprotegerin, PARC, PDGF-BB, PlGF, PPBP, RANTES, SCF, SDF-1 alpha, SPP1, TARC, TCA-3, TCK-1, TGF beta 1, TGF beta 2, TGF beta 3, Thrombopoietin, TIMP-1, TIMP-2, TNF alpha, TNF beta, TNFRSF11B, TNFSF14, TNFSF1B, TPO, VEGF-A
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity
Kit Components
  • Human Cytokine Antibody Array G5 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Glass Slide
Design Principle
Result Output
Detection Method
Fluorescence Laser Scanner
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
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9. Leea Y, Chioub T, Tzengd W, Chua S.Macrophage inflammatory protein-3 influences growth of K562 leukemia cells in co-culture with anticancer drug-pretreated HS-5 stromal cells.Toxicology.2008, 249:116–122
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11. Wong CK, Cheung PFY, Ip WK, Lam CWK. Interleukin-25–Induced Chemokines and Interleukin-6 Release from Eosinophils Is Mediated by p38 Mitogen-Activated Protein Kinase, c-Jun N-Terminal Kinase, and Nuclear Factor-kB. Am J Respir Cell Mol Biol. 2005, 33:186–194.
12. Wong CK, Cheung PFY, Lam CWK. Leptin-mediated cytokine release and migration of eosinophils: Implications for immunopathophysiology of allergic inflammation. Eur J Immunol. 2007, 37:2337–2348.
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17. Hegmans J, Hemmes A, Hammad H, Boon L, Hoogsteden HC, Lambrecht BN. Mesothelioma environment comprises cytokines and Treg cells that suppress immune responses. Eur Respir J. 2006, 27:1086-1095.
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Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 704,05 €
Preis: 704,05 €


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