Human Cytokine Array G5 (4)

Hersteller Raybiotech
Kategorie
Typ Array
Specific against Human
Menge 4 Sample Kit
ArtNr AAH-CYT-G5-4
eClass 6.1 32161000
eClass 9.0 32161000
Lieferbar
Short description
RayBio G-Series Human Cytokine Antibody Array 5 Kit. Detects 80 Human Cytokines. Suitable for all liquid sample types.
Features
  • High sensitivity and specificity
  • Low sample volume (10-100 ul per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays
Number of Targets Detected
80
Target Names
ENA-78 (CXCL5), GCSF, GM-CSF, GRO alpha/beta/gamma, GRO alpha (CXCL1), I-309 (TCA-3/CCL1), IL-1 alpha (IL-1 F1), IL-1 beta (IL-1 F2), IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8 (CXCL8), IL-10, IL-12 p40/p70, IL-13, IL-15, IFN-gamma, MCP-1 (CCL2), MCP-2 (CCL8), MCP-3 (MARC/CCL7), M-CSF, MDC (CCL22), MIG (CXCL9), MIP-1 beta (CCL4), MIP-1 delta (CCL15), RANTES (CCL5), SCF, SDF-1 alpha (CXCL12 alpha), TARC (CCL17), TGF beta 1, TNF alpha, TNF beta (TNFSF1B), EGF, IGF-1, Angiogenin, Oncostatin M, Thrombopoietin (TPO), VEGF-A, PDGF-BB, Leptin, BDNF, BLC (CXCL13), Ck beta 8-1 (CCL23), Eotaxin-1 (CCL11), Eotaxin-2 (MPIF-2/CCL24), Eotaxin-3 (CCL26), FGF-4, FGF-6, FGF-7 (KGF), FGF-9, Flt-3 Ligand, Fractalkine (CX3CL1), GCP-2 (CXCL6), GDNF, HGF, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-16, IP-10 (CXCL10), LIF, Light (TNFSF14), MCP-4 (CCL13), MIF, MIP-3 alpha (CCL20), NAP-2 (PPBP/CXCL7), NT-3, NT-4, Osteopontin (SPP1), Osteoprotegerin (TNFRSF11B), PARC (CCL18), PLGF, TGF beta 2, TGF beta 3, TIMP-1, TIMP-2
Targets Detected
Angiogenin, BDNF, BLC, CCL1, CCL11, CCL13, CCL15, CCL17, CCL18, CCL2, CCL20, CCL22, CCL24, CCL26, CCL4, CCL5, CCL7, CCL8, CK beta 8-1, CX3CL1, CXCL1, CXCL10, CXCL12 alpha, CXCL13, CXCL6, CXCL7, CXCL8, CXCL9, EGF, ENA-78, Eotaxin-1, Eotaxin-2, Eotaxin-3, FGF-4, FGF-6, FGF-7, FGF-9, Flt-3 Ligand, Fractalkine, G-CSF, GCP-2, GDNF, GM-CSF, GRO, GRO alpha, HGF, I-309, IFN-gamma, IGF-1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IL-1 alpha, IL-1 beta, IL-1 F1, IL-1 F2, IL-10, IL-12 p40/p70, IL-13, IL-15, IL-16, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IP-10, KGF, Leptin, LIF, LIGHT, M-CSF, MARC, MCP-1, MCP-2, MCP-3, MCP-4, MDC, MIF, MIG, MIP-1 beta, MIP-1 delta, MIP-3 alpha, MPIF-2, NAP-2, NT-3, NT-4, Oncostatin M, Osteopontin, Osteoprotegerin, PARC, PDGF-BB, PlGF, PPBP, RANTES, SCF, SDF-1 alpha, SPP1, TARC, TCA-3, TCK-1, TGF beta 1, TGF beta 2, TGF beta 3, Thrombopoietin, TIMP-1, TIMP-2, TNF alpha, TNF beta, TNFRSF11B, TNFSF14, TNFSF1B, TPO, VEGF-A
Suggested Applications
Multiplexed Protein Detection, Detection of Relative Protein Expression, Detecting Patterns of Cytokine Expression, Biomarker Screening, Identifying Key Factors, Confirming a Biological Process, Detecting Nephrotoxicity
Kit Components
  • Human Cytokine Antibody Array G5 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis
Storage
For best results, store the entire kit frozen at -20C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20C and all other reagents undiluted at 4C for no more than 3 months.
Solid Support
Glass Slide
Design Principle
Sandwich-based
Result Output
Semi-Quantitative
Detection Method
Fluorescence Laser Scanner
Compatible Sample Types
Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Citation
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2. Zhen A., Krutzik S., Levin B., et al. CD4 ligation on human blood monocytes triggers macrophage differentiation and enhances HIV infection. Journal of Virology published online ahead of print on 18 June 2014. doi:10.1128/JVI.00616-14
3. Hayashi T, Hideshima T, Nguyen A N, Munoz O, Podar K, et al.Transforming Growth Factor Receptor I Kinase Inhibitor Down-Regulates Cytokine Secretion and Multiple Myeloma Cell Growth in the Bone Marrow icroenvironment.Clin Cancer Res. 2004 10, 7540
4. Boucharaba A, Serre C-M, Guglielmi J, Bordet J-C, Clezardin P, Peyruchaud O. The type 1 lysophosphatidic acid receptor is a target for therapy in bone metastases. Proc Natl Acad Sci USA. 2006, 103(25):9643-9648.
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6. Huang RP, Li S, Yang W, Chen L, Yao C, et al. Early Growth Response-1 Suppresses Human Fibrosarcoma Cell Invasion and Angiogenesis.Cancer Genom Proteomics.2006, 3: 71-82
7. Kim M, Oskarsson T, Acharyya S, Nguyen D, Zhang X, et al.Tumor Self-Seeding by Circulating Cancer Cells. Cell. 2009, 139(7):1315–1326.
8. De Ceuninck F, Dassencourt L, Anract P. The inflammatory side of human chondrocytes unveiled by antibody microarrays. Biochem Biophys Res Comm. 2004, 323:960–969.
9. Leea Y, Chioub T, Tzengd W, Chua S.Macrophage inflammatory protein-3 influences growth of K562 leukemia cells in co-culture with anticancer drug-pretreated HS-5 stromal cells.Toxicology.2008, 249:116–122
10. Pislaru SV, Harbuzariu A, Gulati R, Witt T, et al. Magnetically Targeted Endothelial Cell Localization in Stented Vessels. J Am Coll Cardiol. 2006, 48(9):1839-1845.
11. Wong CK, Cheung PFY, Ip WK, Lam CWK. Interleukin-25–Induced Chemokines and Interleukin-6 Release from Eosinophils Is Mediated by p38 Mitogen-Activated Protein Kinase, c-Jun N-Terminal Kinase, and Nuclear Factor-kB. Am J Respir Cell Mol Biol. 2005, 33:186–194.
12. Wong CK, Cheung PFY, Lam CWK. Leptin-mediated cytokine release and migration of eosinophils: Implications for immunopathophysiology of allergic inflammation. Eur J Immunol. 2007, 37:2337–2348.
13. Acosta JC, O'Loghlen A, Banito A, Guijarro MV, Augert A, et al. Chemokine Signaling via the CXCR2 Receptor Reinforces Senescence. Cell. 2008, 133:1006–1018.
14. Yokoyama T, Komori A, Nakamura M, Takii Y, et al. Human intrahepatic bilary epithelial cells function in innate immunity by producing IL-6 and IL-8 via the TLR4-NF-kB and -MAPK signaling pathways. Liver Int. 2006, 26: 467-476.
15. Grossmann C, Podgrabinska S, Skobe M, Ganem D. Activaction of NF-kB by the Latent vFLIP Gene of Kaposi's Sarcoma-Associated Herpesvirus is Required for the Spindle Shape of Virus-Infected Endothelial Cells and Contributes to Their Proinflammatory Phenotype. J Virol. 2006: 80(14):7179-7185.
16. Sivashanmugam P, Tang L, Daaka Y. Interleukin 6 Mediates the Lysophosphatidic Acid-regulated Cross-talk between Stromal and Epithelial Prostate Cancer Cells. J Biol Chem. 2004, 279(20):21154-21150.
17. Hegmans J, Hemmes A, Hammad H, Boon L, Hoogsteden HC, Lambrecht BN. Mesothelioma environment comprises cytokines and Treg cells that suppress immune responses. Eur Respir J. 2006, 27:1086-1095.
18. Wendt K, Wilk E, Buyny S, Buer J, Schmidt RE, Jacobs R. Gene and protein characteristics reflect functional diversity of CD56-dim and CD56-bright NK cells. J Leuk Biol. 2006, 80:1529-1541.
19. Rhee SH, Keates AC, Moyer MP, Pothoulakis C. MEK Is a Key Modulator for TLR5-induced Interleukin-8 and MIP2alpha Gene Expression in Non-transformed Human Colonic Epithelial Cells. J Biol Chem. 2004, 279(24):25179-25188.
20. Boniotto M, Jordan WJ, Eskdale J, Tossi A, Antcheva N, et al. Human beta-Defensin 2 Induces a Vigorous Cytokine Response in Peripheral Blood Mononuclear Cells. Antimicrob Agents Chemother. 2006, 50(4):1433-1441.
21. Chandra J, McCormick TS, Imamura Y, Mukherjee PK, Ghannoum MA.Interaction of Candida albicans with Adherent Human Peripheral Blood Mononuclear Cells Increases C. albicans Biofilm Formation and Results in Differential Expression of Pro- and Anti-Inflammatory Cytokines. Infect Immun. 2007, 75(5):2612–2620.
22. Kastenbauer S, Angele B, Sporer B, Pfister H-W, Koedel U. Patterns of protein expression in infectious meningitis: A cerebrospinal fluid protein array analysis. J Neuroimmunol. 2005, 16:134-139.
23. Krishnan C, Kaplin AI, Graber JS, Darman JS, Kerr DA. Recurrent transverse myelitis following neurobrucellosis: Immunologic features and beneficial response to immunosuppression. J Neurovirol. 2005, 11:225-231.
24. Rupprecht TA, Pfister HW, Angele B, Kastenbauer S, Wilske B, Koedel U. The chemokine CXCL13 (BLC): A putative diagnostic marker for neuroborreliosis. Neurology. 2005, 65:448-450.
25. Kverka M, Burianova J, Lodinova-Zadnikova R, Kocuirkova I, et al. Cytokine Profiling in Human Colostrum and Milk by Protein Array. Clin Chem. 2007, 53(5):955-962.
26. Hegmans J, Hemmes A, Hammad H, Boon L, Hoogsteden HC, Lambrecht BN. Mesothelioma environment comprises cytokines and Treg cells that suppress immune responses. Eur Respir J. 2006, 27:1086-1095.
27. Celis JE, Gromova I, Moreira J M, Cabezon T, et al. Impact of proteomics on bladder cancer research. Pharmacogenomics. 2004, 5(4):381-394.
28. Hashimoto I, Wada J, Hida A, Baba M. Elevated Serum Monoctye Chemoattractant Protein-4 and Chronic Inflammation in Overweight Subjects. Obesity. 2006:14:799-811.
29. Hon K E, Ching G, Wong K, Leung T, Leung A. A Pilot Study to Explore the Usefulness of Antibody Array in Childhood Atopic Dermatitis.JOURNAL OF THE NATIONAL MEDICAL ASSOCIATION. 2008, 500-504
30. Leea Y, Chioub T, Tzengd W, Chua S.Macrophage inflammatory protein-3 influences growth of K562 leukemia cells in co-culture with anticancer drug-pretreated HS-5 stromal cells.Toxicology.2008, 249:116-122
31. Sharma M, Arnason JT, Burt A, Hudson JB. Echinacea Extracts Modulate the Pattern of Chemokine and Cytokine Secretion in Rhinovirus-Infected and Uninfected Epithelial Cells. Phytother Res. 2006, 20:147-152.
32. Chung Y-L, Jian JJ-M, Cheng SH, Tsai SYC, Chuang VP, et al. Sublethal Irradiation Induces Vascular Endothelial Growth Factor and Promotes Growth of Hepatoma Cells: Implications for Radiotherapy of Hepatocellular Carcinoma. Clin Cancer Res. 2006, 12(9):2706-2715.
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Menge: 4 Sample Kit
Lieferbar: In stock
Listenpreis: 704,05 €
Preis: 704,05 €
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