Vergleich

Mouse Pro Somatostatin EIA

ArtNr EIAM-ProSOM-5
Hersteller Raybiotech
Menge 5 Plate Kit
Quantity options 1 Plate Kit 2 Plate Kit 5 Plate Kit
Kategorie
Typ Elisa-Kit
Specific against Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host Hamster - Armenian
Sensitivity 10.9 ng/ml
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Specificity This EIA kit is designed to detect human, mouse, and rat pro-Somatostatin, not active Somatostatin.
Compatible Sample Types
Cell Culture Supernatants
Plasma
Serum
Design Principle
Competition-based
Method of Detection
Colorimetric
Quantitative/Semi-Quantitative
Quantitative
Solid Support
96-well Microplate
Gene Symbols
SMSTSST
Kit Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Standard Peptide
Assay Diluent(s)
Biotinylated Peptide
HRP-Streptavidin
TMB One-Step Substrate
Stop Solution
Assay Diagram
Positive Control Sample
Capture Antibody
User Manual
Other Materials Required
Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation 100 ml and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Orbital shaker
Aluminum foil
Saran Wrap
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Sigma
Plot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
Prepare all reagents, samples and standards as instructed.
Add 100 µl detection antibody to each well.
Incubate 1.5 h at RT or O/N at 4°C.
Add 100 µl standard or sample to each well.
Incubate 2.5 h at RT.
Add 100 µl prepared streptavidin solution.
Incubate 45 min at RT.
Add 100 µl TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl Stop Solution to each well.
Read plate at 450 nm immediately.
Product Features
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Protein Name & Synonyms
Somatostatin (Growth hormone release-inhibiting factor) [Cleaved into: Somatostatin-28
Somatostatin-14]
Manufacturer - Specificity
This EIA kit is designed to detect human, mouse, and rat pro-Somatostatin, not active Somatostatin.
Recommended Dilution
Human: 2x / Mouse: 2x / Rat: 2x
Introduction
Somatostatin, also known as growth hormone-inhibiting hormone (GHIH) that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones. Somatostatin regulates insulin and glucagon.Somatostatin is secreted in several locations in the digestive system: stomach, intestine and delta cells of the pancreas. In the stomach, somatostatin acts on the acid-producing parietal cells via G-coupled receptor to reduce secretion. Somatostatin also indirectly decreases stomach acid production by preventing the release of other hormones including gastrin, secretin and histamine which effectively slows down the digestive process.Somatostatin has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids. In all vertebrates, there exist six different somatostatin genes that have been named SS1, SS2, SS3, SS4, SS5, and SS6. The six different genes along with the five different somatostatin receptors allow somatostatin to possess a large range of functions. Humans have only one somatostatin gene, SST.

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