Mouse Pro Somatostatin EIA

Cell Culture Supernatants
Plasma
Serum

Colorimetric

96-well Microplate

Pre-
Coated 96-well Strip Microplate
Wash Buffer
Standard Peptide
Assay Diluent(s)
Biotinylated Peptide
H
R
P-
Streptavidin
T
M
B One-
Step Substrate
Stop Solution
Assay Diagram
Positive Control Sample
Capture Antibody
User Manual

Prepare all reagents, samples and standards as instructed.
Add 100 µl detection antibody to each well.
Incubate 1.5 h at R
T or O/
N at 4°
C.
Add 100 µl standard or sample to each well.
Incubate 2.5 h at R
T.
Add 100 µl prepared streptavidin solution.
Incubate 45 min at R
T.
Add 100 µl T
M
B One-
Step Substrate Reagent to each well.
Incubate 30 min at R
T.
Add 50 µl Stop Solution to each well.
Read plate at 450 nm immediately.

Somatostatin (Growth hormone release-inhibiting factor) [Cleaved into: Somatostatin-28
Somatostatin-14]

Somatostatin, also known as growth hormone-inhibiting hormone (GHIH) that regulates the endocrine system and affects neurotransmission and cell proliferation via interaction with G protein-coupled somatostatin receptors and inhibition of the release of numerous secondary hormones. Somatostatin regulates insulin and glucagon.Somatostatin is secreted in several locations in the digestive system: stomach, intestine and delta cells of the pancreas. In the stomach, somatostatin acts on the acid-producing parietal cells via G-coupled receptor to reduce secretion. Somatostatin also indirectly decreases stomach acid production by preventing the release of other hormones including gastrin, secretin and histamine which effectively slows down the digestive process.Somatostatin has two active forms produced by alternative cleavage of a single preproprotein: one of 14 amino acids, the other of 28 amino acids. In all vertebrates, there exist six different somatostatin genes that have been named SS1, SS2, SS3, SS4, SS5, and SS6. The six different genes along with the five different somatostatin receptors allow somatostatin to possess a large range of functions. Humans have only one somatostatin gene, SST.