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Human FGFR1 / CD331 Protein (His & GST Tag)

Human FGFR1 / CD331 Protein (His & GST Tag)

Manufacturer	Sino Biological
Category	Oncology Neuroscience Neuroinflammation Metabolism Diabetes Glucose Homeostasis Regulation of Appetite Neural Adhesion Molecules By Cell Type Blastoma Carcinoma Glioma Hematology Leukemia Lymphoma Myeloma Sarcoma Peptides & Proteins Recombinant Proteins Cell Biology Cell Culture Cytokines Astrocytoma By Organ Bladder Cancer
Type	Proteins
Specific against	Human
Amount	20ug
Item no.	10616-H20B1-20
eClass 6.1	34160400
eClass 9.0	42020190
Available
Molecule	FGFR1
Synonym	bFGF-R-1, BFGFR, CD331, CEK, FGFBR, FGFR-1, FLG, FLT-2, FLT2, HBGFR, HH2, HRTFDS, KAL2, N-SAM, OGD
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	Kinases are highly recommended to be shipped at frozen temperature with blue ice or dry ice. Shipment made at ambient temperature may seriously affect the activity of the ordered products.
Protein Construction	A DNA sequence encoding the human FGFR1 isoform 4 (NP_075594.1) cytoplasmic domain (Gly 311-Arg 731) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Expressed Host	Baculovirus-Insect Cells
Purity	> 93 % as determined by SDS-PAGE
Bio-Activity	1. Kinase activity untested 2. Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR1 (aa 311-731) (Cat:10616-H20B1) at 10 ug/ml (100 ul/well) can bind biotinylated human FGF acidic (Cat:10013-HNAE) with a linear range of 15.6-250 ng/ml. 3. Measured by its binding ability in a functional ELISA. Immobilized recombinant human FGFR1 (aa 311-731) (Cat:10616-H20B1) at 10 ug/ml (100 ul/well) can bind biotinylated human FGF basic (Cat:10014-HNAE) with a linear range of 0.16-2.5 ug/ml.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Met
Molecule Mass	The recombinant human FGFR1 (aa 311-731) /GST chimera consists of 658 amino acids and has a calculated molecular mass of 75 KDa. It migrates as an approximately 75 KDa band as predicted in SDS-PAGE under reducing conditions.
Formulation	Supplied as sterile 50mM Tris, 100mM NaCl, pH 8.0, 20% glycerol, 0.3mM DTT
Accession	NP_075594.1
Amino acid sequence	Gly311-Arg731
TAG	N-GST & His

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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10616-H20B1-20-datasheet.pdf

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Amount: 20ug

available

Delivery expected until 6/13/2024