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Recombinant Mouse Erythropoietin R/EPOR Protein (hFc Tag)

Recombinant Mouse Erythropoietin R/EPOR Protein (hFc Tag)

ArtNr	50031-M02B-4
Hersteller	Sino Biological
Menge	4 mg
Quantity options	1 mg 2 mg 4 mg 50 ug
Kategorie	Peptides & Proteins Recombinant Proteins
Typ	Proteins
Specific against	Mouse (Murine, Mus musculus)
ECLASS 10.1	32160409
ECLASS 11.0	32160409
UNSPSC	12352202
Alias	50031-M02B
Similar products	EPOR
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Manufacturer - Category	Protein
Molecule	EPOR
Storage	Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction	A DNA sequence encoding the mouse EPOR (NP_034279.3) (Met1-Pro249) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Expressed Host	Baculovirus-Insect cells
Purity	> 85 % as determined by SDS-PAGE
Bio-Activity	1. Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.05-0.2 ug/mL in the presence of 16 ng/mL Recombinant mouse EPO. 2. Measured by its binding ability in a functional ELISA. 3. Immobilized mouse EPO-His (Cat:51099-M08H) at 10ug/mL (100 uL/well) can bind mouse EPOR-Fc. The EC50 of mouse EPOR-Fc is 0.06-0.13ug/mL.
Endotoxin	< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal	Ala 25
Molecule Mass	The recombinant mouse EPOR/Fc is a disulfide-linked homodimer. The reduced monomer comprises 463 amino acids and has a predicted molecular mass of 51.4 kDa. The apparent molecular mass of the protein is approximately 58.6 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation	Lyophilized from sterile 100 mM Glycine, 10 mM NaCl, pH 7.0.
Accession	NP_034279.3
Amino acid sequence	Met1-Pro249
TAG	C-human IgG1-Fc

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

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50031-M02B-4-datasheet.pdf

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Menge: 4 mg

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