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Recombinant Rat CD6 Protein (His Tag) Europäischer Partner

ArtNr 80311-R08H-1
Hersteller Sino Biological
Menge 1 mg
Quantity options 1 mg 100 ug 2 mg 4 mg
Kategorie
Typ Proteins
Specific against Rat (Rattus norvegicus)
Host Human
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias 80311-R08H
Similar products CD6
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Manufacturer - Category
Protein
Molecule
CD6
Storage
Store it under sterile conditions at -20C to -80C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Protein Construction
A DNA sequence encoding the rat CD6 (Q812A4) (Met1-Asn396) was expressed with a polyhistidine tag at the C-terminus.
Expressed Host
Human Cells
Purity
> 95 % as determined by SDS-PAGE
Bio-Activity
1. Immobilized rat CD6-His at 10 ug/mL (100 uL/well) can bind rat ALCAM-Fc3 (Cat:80221-R02H), The EC50 of rat ALCAM-Fc3 (Cat:80221-R02H) is 19.5-45.5 ng/ml.
2. Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.
When 8 x 104 cells/well are added to ratCD6-His coated plates (10ug/mL, 100 uL/well), approximately 39.2% will adhere after 60 minutes at 37C.
Endotoxin
< 1.0 EU per ug of the protein as determined by the LAL method
Predicted N Terminal
Leu 18
Molecule Mass
The recombinant rat CD6 comprises 390 amino acids and predicts a molecular mass of 42.5 kDa. The apparent molecular mass of the recombinant protein is approximately 64-70 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation
Lyophilized from sterile PBS, pH 7.4.
Accession
Q812A4
Amino acid sequence
Met1-Asn396
TAG
C-His

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 1 mg
Lieferbar: In stock
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