Recombinant Monkeypox Virus Cell Surface-binding Protein, E8L

Proteins|Recombinant Proteins|Viral Proteins

Ambient temperature

Recombinant protein product has a calculated molecular mass of ∼32 kDa. Due to the abundant glycosylation, it migrates as approximately ∼40 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.

SDS-PAGE under reducing conditions and visualized by Coomassie blue staining

12352202

Met1-Thr275

Lyophilized powder

Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.

Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.Lane 1: protein standard ladder (kDa).Lane 2: untreated protein.Lane 3: treated protein with deglycosylation enzymes under native conditions.Lane 4: treated protein with deglycosylation enzymes under denature conditions.