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Human Monocarboxylate transporter 3 (SLC16A8) ELISA Kit

Item no. ABK-KTE60624-96Tx50
Manufacturer Abbkine Scientific
Amount 96 T x 50
Quantity options 48 T 96 T 96 T x 5 96 T x 50
Category
Type Elisa-Kit
Applications ELISA
Specific against Human (Homo sapiens)
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Alias SLC16A8,RP5-1039K5.14,MCT3,REMP,monocarboxylate transporter 3,solute carrier 16 (monocarboxylic acid transporters),member 8,solute carrier family 16,member 8
Shipping Condition Cool pack
Available
Manufacturer - Type
ELISA
Manufacturer - Applications
This Human Monocarboxylate transporter 3 (SLC16A8) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC16A8 in samples. An antibody specific for SLC16A8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC16A8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC16A8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC16A8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Manufacturer - Category
Protein
Manufacturer - Conjugate / Tag
SLC16A8
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Background
SLC16A8 is a member of a family of proton-coupled monocarboxylate transporters that mediate lactate transport across cell membranes .
The deduced 504-amino acid protein has a calculated molecular mass of 52.3 kD. MCT3 has 12 putative transmembrane domains and a long cytoplasmic loop between transmembrane domains 6 and 7. MCT3 shares 85% and 62% identity with rat and chicken Mct3, respectively, and highest similarity is in the transmembrane region. PCR analysis detected MCT3 in RPE RNA, but not in neural retina RNA, suggesting that in human, as in chicken and rat, the expression of MCT3 is restricted to RPE.
Description
This Human Monocarboxylate transporter 3 (SLC16A8) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC16A8.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Human Monocarboxylate transporter 3 (SLC16A8) ELISA Kit has high sensitivity and excellent specificity for detection of Human SLC16A8. No significant cross-reactivity or interference between Human SLC16A8 and analogues was observed.
Usage Notes
• Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Kit components
• Human Monocarboxylate transporter 3 microplate
• Human Monocarboxylate transporter 3 standard
• Human Monocarboxylate transporter 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Limit of detection
Please inquire
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay type
Sandwich ELISA (quantitative)
Calibration range
Please inquire
Detection method
Colorimetric
Sample type
Cell culture supernatants, Serum, Plasma, Other biological fluids
Product Assortment
ELISA Testing
Manufacturer - Search Terms
SLC16A8, RP5-1039K5.14, MCT3, REMP, monocarboxylate transporter 3, solute carrier 16 (monocarboxylic acid transporters), member 8, solute carrier family 16, member 8

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 96 T x 50
Available: In stock
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