EEA1

Manufacturer	Abclonal
Type	Antibody Polyclonal
Specific against	Human
Applications	WB
Protein Family	Vesicle transport
Amount	100ug
Host	Rabbit
Item no.	A0592
eClass 6.1	32160702
eClass 9.0	32160702
Available
*	DESCRIPTION
Calculated MW	162kDa
Immunogen	A synthetic peptide of human EEA1
Gene ID	8411
Swiss Prot	Q15075
Synonyms	MST105; ZFYVE2; MSTP105
Isotype	IgG
Purity	Affinity purification
Storage Buffer	Store at 4? or -20?. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Background	EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes (3). The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin (4-6). Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion (7).
Recommended Dilutions	WB 1:500-1:1000 IHC 1:50-1:100
illustration	Western blot analysis of extracts of ZR-75-1 celllines, using EEA1antibody.

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