Anti-C3aR1 (extracellular)-FITC Antibody

IFC, IHC, LCI, WB

Complement C3a Receptor 1, C3AR, C3a Anaphylatoxin Chemotactic Receptor

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4

Complement C3a Receptor 1, C3AR, C3a Anaphylatoxin Chemotactic Receptor - A Rabbit Polyclonal antibody to C3aR1 (extracellular)

Rat - 13 out of 15 amino acid residues identical It will not recognize C3aR1 from human samples

Confirmed by amino acid analysis and mass spectrometry

0.8 mg/ml

Extracellular, 2nd loop.

Mouse

41116161

The innate immune system identifies pathogens by recognizing known elements. This response ultimately leads to the production of proinflammatory substances and the activation of phagocytic neutrophils and macrophages1. Activation of the complement system is an important initial even in the response against pathogens. This system includes some thirty different proteins that act together to form a complex1.Three different pathways are known to activate the complement. The C3 complement plays a key role in all three pathways leading to the activation of the complement. Produced in the liver, C3 is the most abundant complement protein in the serum. It is composed of two polypeptides, an α and β chain. Cleavage of C3 leads to the production of C3b and C3a1.C3a binds to C3a receptor, a G-protein coupled receptor which couples to Gi upon activation. Like all other members of the superfamily, C3a receptor has seven transmembrane domains with an extracellular N-terminus and intracellular C-terminal tail1. The receptor has two glycosylation sites, an unusually large extracellular loop with a sulfated tyrosine residue important for ligand recognition and binding2, 3.C3a receptor is expressed in cells of myeloid origin like neutrophils, macrophages and dendritic cells to name a few2. It is also detected in astrocytes from inflamed brain4, endothelial cells5 and activated human T cells6. mRNA of C3a receptor is detected in lung, liver, kidney, brain heart, muscle and testis2, 7.