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Anti-P2X7 Receptor (ext)-mFluor Violet 450 Antibody

Anti-P2X7 Receptor (ext)-mFluor Violet 450 Antibody

Item no.	ALO-APR-008-V-5x50ul
Manufacturer	Alomone
Amount	5 x 50 ul
Quantity options	10 x 50 ul 15 ul 2 x 50 ul 50 ul 5 x 50 ul
Category	Neuroscience Neural Lineage Markers Flow Cytometry
Format	Lyophilized
Applications	FC
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Pacific Blue
Purity	Affinity purified on immobilized antigen.
Formula	Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
Sequence	KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor
Shipping Condition	Room temperature
Available
Manufacturer - Type	Antibodies
Manufacturer - Applications	FC, LCI
Manufacturer - Category	Antibodies
Manufacturer - Targets	Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor
Manufacturer - Conjugate / Tag	mFluor™ Violet 450. Maximum absorption 405 nm and maximum emission 445 nm. These spectral characteristics make it an excellent replacement for Pacific Blue™ or Brilliant Violet™ 421 dyes. For flow cytometry applications, the labeled antibody can be detected using the 405 nm laser lane and a filter set of 450/50.
Country of Origin	Israel
Shipping Temperature	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Storage Conditions	Storage before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C. - Storage after Reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
Manufacturer - Format	Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN₃.
Description	Purinergic receptor P2X7, P2RX7, P2Z, ATP receptor - A Rabbit Polyclonal Antibody to P2X7 Receptor conjugated to mFluor™ Violet 450 fluorophore
Clonality	Polyclonal
Homology	Human, rat - identical; bovine - 14/17 amino acid residues identical.
Standard quality control of each lot	Western blot analysis (unlabeled antibody, #APR-008), and direct flow cytometry (labeled antibody).
Reconstitution	15 µl or 50 µl double distilled water (DDW), depending on the sample size.
Antibody Concentration After Reconstitution	1 mg/ml
Specificity	P2RX7
Immunogen source species	Mouse
PH	7, 4
UNSPSC	41116161
Scientific Background	The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7. The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflammatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis.^1-3 Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.⁴

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ALO-APR-008-V-5x50ul-safety-datasheet.pdf