Goat anti-PPAR delta (Isoform 1) Antibody

Pep ELISA, WB

Goat anti-PPAR delta (Isoform 1) Antibody
Principle Name: PPAR delta (Isoform 1)
Official Symbol: PPARD
All Name and Symbols: PPARD; FAAR; NUC1; NUCI; NR1C2; NUCII; PPARB; MGC3931; PPAR-beta; peroxisome proliferative activated receptor, delta; nuclear hormone receptor 1; peroxisome proliferative activated receptor delta; OTTHUMP00000016256; OTTHUMP00000016257
Accession Number(s): NP_006229.1
Peptide Sequence: CHPLLQEIYKDMY, is from C Terminus
Human Gene ID(s): 5467
Important Comments: This antibody is expected to recognise isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 32000.
Western Blot: Approx 55kDa band observed in T cell line MOLT4 lysates (calculated MW of 49.9kDa according to NP_006229.1). Recommended concentration: 0.3ug/ml.