Goat anti-FACT / SUPT16H Antibody

Goat anti-FACT / SUPT16H Antibody
Principle Name: FACT / SUPT16H
Official Symbol: SUPT16H
All Name and Symbols: SUPT16H; FACT; suppressor of Ty 16 homolog (S. cerevisiae); HGNC:11465; CDC68; FACTP140; FLJ10857; FLJ14010; SPT16/CDC68; chromatin-specific transcription elongation factor large subunit; FLJ34357; facilitates chromatin remodeling 140 kDa subunit
Accession Number(s): NP_009123.1
Peptide Sequence: DLTNKEGKKPEEK, is from internal region
Human Gene ID(s): 11198
Non-Human GeneID(s):
Important Comments:
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB
Tested Species: Human
Peptide ELISA: antibody detection limit dilution 1 to 8000.
Western Blot: Approx 150kDa band observed in lysates of cell line HeLa (calculated MW of 120kDa according to NP_009123.1). Recommended concentration: 0.1-0.3ug/ml.
An additional band of unknown identity was also consistently observed at 30kDa. This band
Reference(s): Belotserkovskaya R, Oh S, Bondarenko VA, Orphanides G, Studitsky VM, Reinberg D. FACT facilitates transcription-dependent nucleosome alteration. Science. 2003 Aug 22; 301(5636):1090-3. .PMID: 12934006