Goat anti-DLG4 / PSD95 Antibody

Pep ELISA, WB

Goat anti-DLG4 / PSD95 Antibody
Principle Name: DLG4 / PSD95
Official Symbol: DLG4
All Name and Symbols: DLG4; discs, large homolog 4 (Drosophila); FLJ97752; FLJ98574; PSD95; SAP90; Tax interaction protein 15; discs large homolog 4; post-synaptic density protein 95; synapse-associated protein 90
Accession Number(s): NP_001356.1; NP_001122299.1
Peptide Sequence: EEQARKAFDRATK, is from internal region (near C-Terminus)
Human Gene ID(s): 1742
Non-Human GeneID(s): 13385 (mouse); 29495 (rat);
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001356.1; NP_001122299.1)
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 64000.
Western Blot: Approx 110kDa band observed in Rat Brain lysates (calculated MW of 80.5kDa according to Rat NP_062567.1).The observed molecular weight corresponds to earlier findings in literature with different antibodies (Chaudhury et al, , Am J Physiol
Reference(s): Marks DR, Fadool DA, Post-synaptic density perturbs insulin-induced Kv1.3 channel modulation via a clustering mechanism involving the SH3 domain. Journal of neurochemistry 2007 Nov 103 (4): 1608-27.PMID: 17854350