Goat anti-CD11A / integrin alpha L Antibody

Goat anti-CD11A / integrin alpha L Antibody
Principle Name: CD11A / integrin alpha L
Official Symbol: ITGAL
All Name and Symbols: ITGAL; integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide); CD11A; LFA-1; LFA1A; LFA-1 alpha; antigen CD11A (p180), lymphocyte function-associated antigen 1, alpha polypeptide; integrin alpha L; integrin g
Accession Number(s): NP_002200.2; NP_001107852.1
Peptide Sequence: YQVRIQPSIHDHN, is from internal region
Human Gene ID(s): 3683
Important Comments: This antibody is expected to recognize both reported isoforms (NP_002200.2; NP_001107852.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA, WB, IHC
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 200kDa band observed in Human Spleen and Tonsil lysates (calculated MW of 129kDa according to NP_002200.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recomm
Immunohistochemistry: In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Spleen. Recommended concentration: 3.75ug/ml.
Reference(s): Stanley P, Smith A, McDowall A, Nicol A, Zicha D, Hogg N, Intermediate-affinity LFA-1 binds alpha-actinin-1 to control migration at the leading edge of the T cell. The EMBO journal 2008 Jan 27 (1): 62-75.PMID: 18079697