Goat anti-NPR3 (aa520-533) Antibody

Pep ELISA, WB, IHC

Goat anti-NPR3 (aa520-533) Antibody
Principle Name: NPR3 (aa520-533)
Official Symbol: NPR3
All Name and Symbols: NPR3; natriuretic peptide receptor C/guanylate cyclase C (atrionatriuretic peptide receptor C); ANPRC; GUCY2B; NPRC; ANP-C; ANPR-C; NPR-C; OTTHUMP00000197048; OTTHUMP00000220980; atrial natriuretic peptide clearance receptor; atrial natriuretic peptide re
Accession Number(s): NP_000899.1
Peptide Sequence: HRELREDSIRSHFS, is from C Terminus
Human Gene ID(s): 4883
Non-Human GeneID(s): 18162 (mouse); 25339 (rat);
Important Comments: The immunizing peptide represents the cytoplasmic part of the protein.
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 16000.
Western Blot: Approx 52kDa band observed in Human Placenta lysates (calculated MW of 59.8kDa according to NP_000899.1). Recommended concentration: 1-3ug/ml.
Immunohistochemistry: In paraffin embedded Human Kidney shows staining of brush borders in select PCT. Recommended concentration: 2-4ug/ml. Paraffin embedded Human Prostate. Recommended concentration: 10ug/ml.
Reference(s): Shibasaki I, Nishikimi T, Mochizuki Y, Yamada Y, Yoshitatsu M, Inoue Y, Kuwata T, Ogawa H, Tsuchiya G, Ishimitsu T, Fukuda H. Greater expression of inflammatory cytokines, adrenomedullin, and natriuretic peptide receptor-C in epicardial adipose tissue in coronary artery disease. Regul Pept. 2010 Dec 10; 165(2-3):210-7.PMID: 20691218