Goat anti-Alfy / WDFY3 Antibody

Pep ELISA, WB

Goat anti-Alfy / WDFY3 Antibody
Principle Name: Alfy / WDFY3
Official Symbol: WDFY3
All Name and Symbols: WDFY3; WD repeat and FYVE domain containing 3; ALFY; ZFYVE25; WD repeat and FYVE domain-containing protein 3; autophagy-linked FYVE protein
Accession Number(s): NP_055806.2
Peptide Sequence: SPERSTRTQQKEFQT, is from internal region
Human Gene ID(s): 23001
Non-Human GeneID(s): 72145 (mouse); 305164 (rat);
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Approx 400kDa band observed in Mouse Brain lysates (calculated MW of 395kDa according to NP_055806.2). Data obtained from anonymous customer. The lower molecular weight bands are non-specific. We call for caution when used for other assays
Reference(s): Yamamoto A, Simonsen A. Alfy-dependent elimination of aggregated proteins by macroautophagy: can there be too much of a good thing? Autophagy 2011 Mar 7 (3): 346-50.PMID: 21150266