Goat anti-CENPE (aa2363-2374) Antibody

Goat anti-CENPE (aa2363-2374) Antibody
Principle Name: CENPE (aa2363-2374)
Official Symbol: CENPE
All Name and Symbols: CENPE; centromere protein E, 312kDa; CENP-E; KIF10; PPP1R61; Centromere autoantigen E (312kD); centromere-associated protein E; kinesin family member 10; kinesin-related protein CENPE; protein phosphatase 1, regulatory subunit 61
Accession Number(s): NP_001804.2; NP_001273663.1
Peptide Sequence: TQDNKNPHVTSR, is from internal region
Human Gene ID(s): 1062
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001804.2; NP_001273663.1).
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As: lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20C. Minimize freezing and thawing.
Applications: Pep ELISA
Peptide ELISA: antibody detection limit dilution 1 to 128000.
Western Blot: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 316kDa according to NP_001804.2. Therefore we cannot recommend an optimal concentration and the antibody i
Reference(s): Sardar HS, Gilbert SP. Microtubule capture by mitotic kinesin centromere protein E (CENP-E). The Journal of biological chemistry 2012 Jul 287 (30): 24894-904.PMID: 22637578