Mouse Monoclonal Antibody to SMC1

Mouse IgG1

MW: 143kDa
Enterez GeneID: 8243
General Information: Proper cohesion of sister chromatids is a prerequisite for the correct segregation of chromosomes during cell division. The cohesin multiprotein complex is required for sister chromatid cohesion. This complex is composed partly of two structural maintenance of chromosomes (SMC) proteins, SMC3 and either SMC1L2 or the protein encoded by this gene. Most of the cohesin complexes dissociate from the chromosomes before mitosis, although those complexes at the kinetochore remain. Therefore, the encoded protein is thought to be an important part of functional kinetochores. In addition, this protein interacts with BRCA1 and is phosphorylated by ATM, indicating a potential role for this protein in DNA repair. This gene, which belongs to the SMC gene family, is located in an area of the X-chromosome that escapes X inactivation.
Immunogen: Purified recombinant fragment of human SMC1 expressed in E. Coli. ;
Size and Concentration: 100ug/1mg/ml
Supplied As: Lyophilized Powder from 100ul of Purified antibody in PBS with 0.05% sodium azide
Reconstitution and Storage: Reconstituted with 100ul sterile DI H2O, at stored at 4C or -20C for short or long term storage.
Applications: ELISA: 1 to 10000; WB: 1 to 500 - 1 to 2000; IHC: 1 to 200 - 1 to 1000; ICC: 1 to 200 - 1 to 1000; FCM: 1 to 200 - 1 to 400
References: 1. Biochem Biophys Res Commun. 2008 Dec 19; 377(3):852-6. ; 2. Curr Top Med Chem. 2009; 9(3):235-40. ; 3. Nucleic Acids Res. 2010 May; 38(9):2813-24.