Description |
Product Name: Keratinocyte Growth Factor-2 Rat Recombinant Synonyms: FGFA, FGF10, FGF-10, KGF-2, Fibroblast growth factor 10. Description: KGF 2 Rat Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 179 amino acids and having a molecular mass of 20.0kDa. The KGF 2 is purified by proprietary chromatographic techniques. Uniprot Accesion Number: P70492 Amino Acid Sequence:QALGQDMVSP EATNSSSSSS SSSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS Source: Escherichia Coli. Physical Appearance and Stability: Lyophilized KGF 2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution KGF 2 should be stored at 4C between 2-7 days and for future use below -18C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. Formulation and Purity: Lyophilized from a 0.2um filtered concentrated solution in 1xPBS, pH 7.4 containing 5% trehalose. Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. Application: Solubility: It is recommended to reconstitute the lyophilized KGF 2 Rat Recombinant in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is < 0.5ng/ml. Shipping Format and Condition: Lyophilized powder at room temperature. Usage Statement: Our products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals. |