Anti-IGSF8 (extracellular) Antibody

Antibodies

Antibodies

Israel

Storage before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C - Storage after Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Immunoglobulin superfamily member 8, Prostaglandin Regulatory-Like Protein, PGRL, Glu-Trp-Ile EWI motif-containing protein 2, EWI2, CD81 Partner 3, KCT4, CD316 - A Rabbit polyclonal antibody to IGSF8 (extracellular)

Human–15/16 identical, Rat–14/16 identical

Confirmed by amino acid analysis and mass spectrometry

0.8 mg/ml

Extracellular, N-terminus.

Mouse

41116161

IGSF8, also referred to as EWI-2, CD316, and PGRL, is a member of the immunoglobulin superfamily (IgSF), which is characterized by extracellular immunoglobulin-like domains. IGSF8 specifically contains four such domains along with a short cytoplasmic tail, enabling its roles in cell-cell adhesion, intracellular signaling, and the organization of cell surface microdomains1, 2. It associates with tetraspanins, such as CD9 and CD81, forming the tetraspanin web, a molecular network that organizes cell-surface proteins into functional microdomains and regulates processes like adhesion, migration, and signaling3. Furthermore, IGSF8 interacts with the ezrin-radixin-moesin (ERM) protein family, linking the tetraspanin web to the actin cytoskeleton and contributing to cell polarity and motility4, 5. Functionally, IGSF8 acts as an immune checkpoint by suppressing natural killer (NK) cell cytotoxicity through interactions with receptors like KIR3DL2, allowing tumor cells to evade immune detection3. Its expression correlates with poor outcomes in cancer and low antigen presentation, making it a promising target for therapeutic intervention3, 4. Beyond its role in immune modulation, IGSF8 contributes to reproductive biology by participating in sperm-egg fusion2. IGSF8 is expressed across various tissues, including neural and immune cells1. Disruption of its function enhances cellular migration and inhibits metastasis4, highlighting its therapeutic relevance. As a cancer immunotherapy target, IGSF8 has shown potential, particularly when combined with immune checkpoint inhibitors like anti-PD1 antibodies3. Research into IGSF8 is critical for understanding tumor immune evasion, immunotherapeutic development, and its roles in neural and reproductive biology1, 5.