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Anti-CD33 antibody(DM77); Rabbit mAb

Anti-CD33 antibody(DM77); Rabbit mAb

Item no.	DIM-DME100077-10ug
Manufacturer	DIMA Biotechnology
Amount	10 ug
Quantity options	100 uL 100 ug 10 uL 10 ug 500 ug
Category	Oncology Neuroscience Neuroinflammation Antibodies By Cell Type Hematology Leukemia Myeloma Flow Cytometry ELISA Primary Antibodies
Type	Antibody Primary
Format	Lyophilized
Applications	FC, ELISA
Clone	DM77
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	CD33, SIGLEC3, gp67
Shipping condition	Room temperature
Available
Manufacturer - Category	Monoclonal antibodies
Manufacturer - Targets	CD33
Molecular Weight	39.8kDa
Buffer	50% Glycerol; PBS, pH 7.4; 0.1% BSA
Clonality	Monoclonal
Purification	Purified from cell culture supernatant by affinity chromatography
Recommended Dilutions	ELISA 1:5000-10000; Flow Cyt 1:100
Storage	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Background	Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2, 3- and more avidly alpha-2, 6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins; two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6:SHP-1 and PTPN11:SHP-2. In turn; these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase:PI3K.
Immunogen	Recombinant human CD33 (Asp18-His269) (PME100039) produced by using human HEK293 cells
Antigen	CD33
Application Advice	ELISA 1/5000-10000; Flow Cyt 1/100
Description	Anti-CD33 antibody(DM77); Rabbit mAb
Preservative	0.1% Procline 300
Reconstitution	Reconstitute with deionized water
Formulation & Reconstitution	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Usage	Research use only

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 10 ug

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Delivery expected until 7/31/2025

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