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RYK Antibody

RYK Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Isotype	IgG1 kappa
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Applications	WB, FC
Amount	200 ul
Host	Mouse
Item no.	ABC-AM8543b
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	P34925
Antigen Type	Recombinant Protein
Application	WB, FC
Bio Background	May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C- terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development.
Bio References	Stacker S.A., et al.Oncogene 8:1347-1356(1993). Tamagnone L., et al.Oncogene 8:2009-2014(1993). Wang X.C., et al.Mol. Med. 2:189-203(1996). Katso R.M., et al.Mol. Cell. Biol. 19:6427-6440(1999). Lu W., et al.Cell 119:97-108(2004).
Clonality	monoclonal
Clone/Animal Names	1671CT575.42.61
Gene ID	6259
Gene Name	RYK (HGNC:10481)
Subtitle	Purified Mouse Monoclonal Antibody (Mab)
Reactivity	H
Legend image 1	Anti-RYK Antibody at 1:1000 dilution + human lung lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 1	WB
Dilution image 1	1:1000
Legend image 2	Overlay histogram showing A431 cells stained with AM8543b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AM8543b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
Type image 2	FC
Dilution image 2	1:25
Isotype	IgG1, k
Calculated MW	67815
Antigen Source	HUMAN
Target/Specificity	This RYK antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 260-565 amino acids from human RYK.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-AM8543b-datasheet.pdf

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Amount: 200 ul

available

Delivery expected until 7/4/2024