Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Primary |
Specific against |
other |
Isotype |
Ig |
Format |
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Applications |
WB |
Amount |
400 ul |
Host |
Rabbit |
Item no. |
ABC-AP17107b |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Primary Accession |
O14682 |
Antigen Type |
Synthetic Peptide |
Application |
WB |
Bio Background |
DNA damage and/or hyperproliferative signals activatewildtype p53 tumor suppressor protein (TP53; MIM 191170), inducingcell cycle arrest or apoptosis. Mutations that inactivate p53 occurin 50% of all tumors. Polyak et al. (1997) [PubMed 9305847] usedserial analysis of gene expression (SAGE) to evaluate cellular mRNAlevels in a colorectal cancer cell line transfected with p53. Of7, 202 transcripts identified, only 14 were expressed at levels morethan 10-fold higher in p53-expressing cells than in control cells.Polyak et al. (1997) [PubMed 9305847] termed these genes'p53-induced genes, ' or PIGs, several of which were predicted toencode redox-controlling proteins. They noted that reactive oxygenspecies (ROS) are potent inducers of apoptosis. Flow cytometricanalysis showed that p53 expression induces ROS production, whichincreases as apoptosis progresses under some conditions. Theauthors stated that the PIG10 gene, also called ENC1, encodes anactin-binding protein. |
Bio References |
Seng, S., et al. Oncogene 28(3):378-389(2009)Wang, X.J., et al. PLoS ONE 4 (5), E5492 (2009) :Seng, S., et al. Cancer Res. 67(18):8596-8604(2007)Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005)Kim, T.A., et al. Gene 255(1):105-116(2000) |
Clonality |
Polyclonal |
Clone/Animal Names |
RB37065 |
Gene ID |
8507 |
Gene Name |
ENC1 |
Subtitle |
Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Reactivity |
H |
Legend image 1 |
ENC1 Antibody (C-term) (Cat. #AP17107b) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ENC1 antibody detected the ENC1 protein (arrow). |
Type image 1 |
WB |
Dilution image 1 |
1:1000 |
Other Accession |
NP_003624.1 |
Isotype |
Rabbit Ig |
Calculated MW |
66130 |
Antigen Region |
395-423 |
Antigen Source |
HUMAN |
Target/Specificity |
This ENC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 395-423 amino acids from the C-terminal region of human ENC1. |
NCBI Accession |
NP_001243503.1; NP_001243504.1; NP_001243505.1; NP_003624.1 |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.