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PRG4 Antibody (N-Term)

PRG4 Antibody (N-Term)

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Isotype	Ig
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Applications	WB, IF
Amount	200 ul
Host	Rabbit
Item no.	ABC-AP22357a
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	Q92954
Antigen Type	Synthesized Peptide
Application	WB, IF
Bio Background	Plays a role in boundary lubrication within articulating joints. Prevents protein deposition onto cartilage from synovial fluid by controlling adhesion-dependent synovial growth and inhibiting the adhesion of synovial cells to the cartilage surface.
Bio References	Turner K.J., et al.Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases. Ota T., et al.Nat. Genet. 36:40-45(2004). Gregory S.G., et al.Nature 441:315-321(2006). Jones A.R., et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases. Flannery C.R., et al.Biochem. Biophys. Res. Commun. 254:535-541(1999).
Clonality	polyclonal
Clone/Animal Names	RB57887
Gene ID	10216
Gene Name	PRG4
Subtitle	Purified Rabbit Polyclonal Antibody (Pab)
Reactivity	H
Legend image 1	Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (human cervical epithelial adenocarcinoma cell line) cells labeling PRG4 with AP22357a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
Type image 1	IF
Dilution image 1	1:25
Legend image 2	Anti-PRG4 Antibody (N-Term) at 1:500 dilution + Human liver whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 151 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Type image 2	WB
Dilution image 2	1:500
Predicted	H
Isotype	Rabbit Ig
Calculated MW	151061
Antigen Source	HUMAN
Target/Specificity	This PRG4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 176-210 amino acids from the human region of human PRG4.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-AP22357a-datasheet.pdf

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Amount: 200 ul

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Delivery expected until 7/4/2024