AIFM1 Antibody (N-term)

O95831

FC, IHC-P, WB, IF

References for protein:
1.Daugas, E., et.al., FASEB J. 14 (5), 729-739 (2000)
2.Schulthess, F.T., et.al., PLoS ONE 4 (2), E4394 (2009)
References for U251 cell line:
1. Westermark B.; Pontén J.; Hugosson R. (1973).” Determinants for the establishment of permanent tissue culture lines from human gliomas”. Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. Pontén, J., Westermark B. (1978).” Properties of Human Malignant Glioma Cells in Vitro”. Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.; Kohli L.; Klocke B.J.; Roth K.A.(2010). “Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent”. Neuro Oncol. 12(5): 473–481.[ PMID: 20406898].

RB22966

AIFM1 (HGNC:8768)

H

IF

Fluorescent confocal image of U251 cells stained with AIFM1 (N-term) antibody. U251 cells were treated with Chloroquine (50 μM, 16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AP8910a AIFM1 (N-term) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). AIFM1 (N-term) immunoreactivity is localized to the cytoplasm of U251 cells.

1:200

WB

Formalin-fixed and paraffin-embedded human brain tissue reacted with AIFM1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

1:50~100

FC

Rabbit Ig

70-98

This AIFM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 70-98 amino acids from the N-terminal region of human AIFM1.