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HSF1 Antibody

HSF1 Antibody

Manufacturer	Abcepta
Category	Antibodies Primary Antibodies
Type	Antibody Primary
Specific against	other
Format	HRP
Amount	Inquiry
Item no.	ABC-ASM10028-HRP-100
eClass 6.1	32160702
eClass 9.0	32160702
Available
Primary Accession	P38532
Reactivity	H, M, Rat, Rb, Ha, Pr, B, Gp
Legend image 1	Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (ASM10028). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10028) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite.
Type image 1	ICC/IF
Dilution image 1	1:200
Legend image 2	Immunohistochemistry analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (ASM10028). Tissue: Breast carcinoma. Species: Human. Fixation: 10% Formalin Solution for 20 hours at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10028) at 1:1000 for 40 min. Secondary Antibody: Dako labeled Polymer HRP Anti-rat IgG, DAB Chromogen (brown) (Dako Envision+ System) for 30 min at RT. Counterstain: Mayer's Hematoxylin (purple/blue) nuclear stain for 1 minute at RT. Localization: Nuclear. Magnification: 100X. Courtesy of: Dr. Sandro Santagata, Harvard Medical School.
Type image 2	IHC
Dilution image 2	1:1000
Legend image 3	Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (ASM10028). Tissue: Heat Shocked mitotic HeLa cells. Species: Human. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10028) at 1:1000. Courtesy of: Morimoto Lab, Northwestern University, USA.
Type image 3	WB
Legend image 4	Western Blot analysis of Human Heat Shocked HeLa cell lysates showing detection of ~85-95 kDa HSF1 protein using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (ASM10028). Lane 1: MW ladder. Lane 2: HeLa cell lysates (Control). Lane 3: Heat-shocked HeLa cell lysates. Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10028) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT. Predicted/Observed Size: ~85-95 kDa.
Type image 4	WB
Dilution image 4	1:1000
Legend image 5	Immunocytochemistry/Immunofluorescence analysis using Rat Anti-HSF1 Monoclonal Antibody, Clone 10H8 (ASM10028). Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-HSF1 Monoclonal Antibody (ASM10028) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Diffuse nuclear and cytoplasmic staining. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-HSF1 Antibody. (C) Composite.
Type image 5	ICC/IF
Dilution image 5	1:200
Other Accession	NP_032322.1
Isotype	IgG1
Target/Specificity	Detects ~85kDa (unstressed cell lysates), and~95kDa (heat shocked cell lysates).
Immunogen	Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395
Purification	Protein G Purified
Reconstitution & Storage	-20ºC

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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ABC-ASM10028-HRP-100-datasheet.pdf

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Delivery expected until 7/4/2024