Manufacturer |
Abcepta
|
Category |
|
Type |
Antibody Primary |
Specific against |
other |
Clone |
200000 |
Format |
Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
Applications |
WB, IF |
Amount |
100 ug |
Host |
Mouse |
Item no. |
ABC-AT3441a |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Primary Accession |
Q9UMS4 |
Antigen Type |
Recombinant Protein |
Application |
WB, IF |
Bio Background |
PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]). |
Bio References |
Regulation of Metnase's TIR binding activity by its binding partner, Pso4. Beck BD, et al. Arch Biochem Biophys, 2010 Jun 15. PMID 20416268.Molecular architecture of the human Prp19/CDC5L complex. Grote M, et al. Mol Cell Biol, 2010 May. PMID 20176811.Blom7alpha is a novel heterogeneous nuclear ribonucleoprotein K homology domain protein involved in pre-mRNA splicing that interacts with SNEVPrp19-Pso4. Grillari J, et al. J Biol Chem, 2009 Oct 16. PMID 19641227.Human Pso4 is a metnase (SETMAR)-binding partner that regulates metnase function in DNA repair. Beck BD, et al. J Biol Chem, 2008 Apr 4. PMID 18263876.Large-scale mapping of human protein-protein interactions by mass spectrometry. Ewing RM, et al. Mol Syst Biol, 2007. PMID 17353931. |
Clonality |
Monoclonal |
Gene ID |
27339 |
Gene Name |
|
Subtitle |
Mouse monoclonal antibody raised against a partial recombinant PRPF19. |
Reactivity |
H, M, Rat |
Legend image 1 |
Immunofluorescence of monoclonal antibody to PRPF19 on HeLa cell . [antibody concentration 10 ug/ml] |
Type image 1 |
IF |
Legend image 2 |
Antibody Reactive Against Recombinant Protein.Western Blot detection against Immunogen (35.64 KDa) . |
Type image 2 |
WB |
Dilution image 2 |
1:500~1000 |
Legend image 3 |
PRPF19 monoclonal antibody (M07), clone 2E5. Western Blot analysis of PRPF19 expression in PC-12((Cat # AT3441a ) |
Type image 3 |
WB |
Dilution image 3 |
1:500~1000 |
Legend image 4 |
PRPF19 monoclonal antibody (M07), clone 2E5. Western Blot analysis of PRPF19 expression in Hela S3 NE ( (Cat # AT3441a ) |
Type image 4 |
WB |
Dilution image 4 |
1:500~1000 |
Legend image 5 |
PRPF19 monoclonal antibody (M07), clone 2E5. Western Blot analysis of PRPF19 expression in NIH/3T3((Cat # AT3441a ) |
Type image 5 |
WB |
Dilution image 5 |
1:500~1000 |
Legend image 6 |
Western Blot analysis of PRPF19 expression in transfected 293T cell line by PRPF19 monoclonal antibody (M07), clone 2E5.
Lane 1: PRPF19 transfected lysate(55.2 KDa). Lane 2: Non-transfected lysate. |
Type image 6 |
WB |
Dilution image 6 |
1:500~1000 |
Legend image 7 |
Detection limit for recombinant GST tagged PRPF19 is 0.3 ng/ml as a capture antibody. |
Type image 7 |
E |
Other Accession |
NM_014502 |
Isotype |
IgG2a Kappa |
Antigen Source |
Human: ~~ Positive |
Target/Specificity |
PRPF19 (NP_055317, 1 a.a. ~ 90 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
NCBI Accession |
NP_055317.1 |
|
Clone: 200000 |
Org Accession |
NP_055317 |
Gene Description |
PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae) |
Gene Summary |
PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]).[supplied by OMIM |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.