Anti-Beta2-Adrenoceptor (extracellular)-FITC

FC, LCI

Beta-2 adrenoceptor, Beta-2 adrenoreceptor, ADRB2, B2AR

Israel

Storage before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C - Storage after Reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

A Rabbit Polyclonal Antibody to β2-Adrenergic Receptor Conjugated to the Fluorescent Dye FITC

Polyclonal

Western blot analysis (unlabeled antibody, #AAR-016), and direct flow cytometry (labeled antibody).

50 µl double distilled water (DDW).

1% BSA, 0.05% NaN3

ADRB2

7, 4

yes

G-protein coupled receptors (GPCRs) comprise the largest protein superfamily in mammalian genomes. Signal transduction by GPCRs is fundamental for most physiological processes, spanning from vision, smell and taste to neurological, cardiovascular, endocrine, and reproductive functions, thus, making the GPCR superfamily a major target for therapeutic intervention.The β2-adrenergic receptor (ADRB2) is comprised of seven transmembrane α-helices, connected by three extracellular loops and three intracellular loops. The extracellular part, responsible for ligand binding, also includes the N-terminus. The intracellular C-terminal interacts with G-proteins, arrestins and other downstream effectors1.Unlike some GPCRs, ADRB2 activates more than one G-protein and signals through at least one known G-protein-independent pathway, arrestin. In addition, there is a rich diversity of available ligands for ADRB2. These ligands are often characterized as inverse agonists that suppress basal activity, full agonists that maximally activate the receptor, partial agonists that produce submaximal activity even at saturating concentrations, and neutral antagonists that occupy the orthosteric binding site but do not affect basal activity2.ADRB2 located on the surface of cardiomyocytes, mediates distinct effects on cardiac function and the development of heart failure by regulating production of the secondary messenger cyclic adenosine monophosphate (cAMP). In cardiomyocytes from healthy adult rats and mice, spatially confined ADRB2 induces cAMP signals that are localized exclusively to the deep transverse tubules. In contrary, in cardiomyocytes derived from a rat model of chronic heart failure, ADRB2 is redistributed from the deep transverse tubules to the cell crest, which leads to diffuse receptor-mediated cAMP signaling. Thus, the redistribution of ADRB2 in heart failure might contribute to the failing myocardial phenotype3.