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Anti-Bombesin Receptor 3 (extracellular)

Anti-Bombesin Receptor 3 (extracellular)

Item no.	ALO-ABR-003-0.2ml
Manufacturer	Alomone
Amount	0.2 ml
Quantity options	0.2 ml 50 ul
Category	Oncology Metabolism Glucose Homeostasis Antibodies Immunohistochemistry Western Blot Primary Antibodies By Organ Lung Cancer
Type	Antibody Polyclonal
Format	Lyophilized
Applications	WB, IHC
Specific against	Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus)
Host	Rabbit
Isotype	IgG
Conjugate/Tag	Unconjugated
Purity	Affinity purified on immobilized antigen.
Formula	PBS pH7.4, 1% BSA with 0.05% sodium azide
Sequence	(C)SNVYTFRDPNKNMTFE, corresponding to amino acid residues 186-201 of human BRS3
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping Condition	Room temperature
Available
Specificity	Polyclonal
Manufacturer - Type	Antibodies
Manufacturer - Applications	IFC, IHC, LCI, WB
Manufacturer - Category	Antibodies
Manufacturer - Targets	BB3R, BRS3
Country of Origin	Israel
Shipping Temperature	Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
Storage Conditions	Storage before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C - Storage after Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Manufacturer - Format	Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4
Short description	A Rabbit Polyclonal Antibody to Bombesin Receptor 3
Description	BB3R, BRS3 - A Rabbit Polyclonal Antibody to Bombesin Receptor 3
Clonality	Polyclonal
Homology	Rat, mouse - 13/16 amino acids residues identical
Standard quality control of each lot	Western blot analysis
Peptide confirmation	Confirmed by amino acid analysis and mass spectrometry
Reconstitution	50 μl or 0.2 ml double distilled water (DDW), depending on the sample size.
Antibody Concentration After Reconstitution	0.8 mg/ml
Preservative	1% BSA, 0.05% NaN3
Immunogen Location	2nd extracellular loop
Specificity	BRS3
Immunogen source species	Human
PH	7, 4
UNSPSC	41116161
Antigen Preadsorption Control	1 µg peptide per 1 µg antibody
Scientific Background	Bombesin receptor 3 (BB3) is a member of a family of receptors that binds the 14 amino acid peptide bombesin. Bombesin was first isolated from frog skin and it was later established that mammalians express endogenous bombesin like peptides such as gastrin-releasing peptide (GRP) a 27 amino acid homologue and neuromedin B (NMB) a 10 amino acid homologue.1BB1 is the preferred receptor for NMB while BB2 is the preferred receptor for GRP.BB3 was the third member of the bombesin receptors family to be cloned based in its sequence similarity to BB1 and BB2.2 The affinity of BB3 for bombesin is lower than that of the BB1 and BB2 receptors as is its affinity for NMB and GRP. This suggests that the mammalian endogenous ligand for the BB3 receptor remains to be identified.All three bombesin receptors are members of the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. BB3 is coupled to a Gq/11 protein that activates phospholipase C (PLC) and leads to production of inositol 1, 4, 5-trisphosphate (InsP3), intracellular Ca2+ mobilization and cell growth.3The physiological function of the BB3 receptor is not clear. However, studies with BB3 receptor deficient mice have shown that the mice develop mild obesity associated with hypertension and reduced metabolic rate.4, 5Finally, BB3 receptor expression has been identified in several human tumors most notably lung. BB3 is therefore considered a potential target for the development of both diagnostics and anti-cancer therapies.6

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

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ALO-ABR-003-0.2ml-safety-datasheet.pdf