Anti-CaV2.3

Antibodies

Israel

Storage before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C - Storage after Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

A Rabbit Polyclonal Antibody to CaV2.3 (CACNA1E) Channel

Polyclonal

Western blot analysis

25 µl, 50 µl or 0.2 ml double distilled water (DDW), depending on the sample size.

1% BSA, 0.05% NaN3

CACNA1E

7, 4

yes

Voltage-dependent Ca2+ channels (CaV channels) are pivotal players in many physiological roles such as secretion, contraction, migration and excitation.1The voltage-dependent Ca2+ channels are composed of several subunits; α1, β, α2δ and γ. CaV channels were originally divided into six physiological types: L-, N-, P-, Q-, R-, and T-type.1The CaV2.3 (formerly named α1E) protein, forms the R-type channels. R-type channels are highly sensitive to both SNX-482 (#RTS-500) and NiCl2. CaV2.3 channels were shown to mediate fast excitatory transmission in several synapses including the hippocampus.2CaV2.3 channels can be modulated by the muscarinic G-protein coupled receptors. In HEK-293 cells, expressing CaV2.3 channels, the R-type channel was shown to be both facilitated and inhibited by m1 and m2 muscarinic receptors. However, inhibition was much weaker than facilitation for the m1 receptor, while for m2 receptors, inhibition was much more pronounced than activation.3Facilitation and inhibition by the same receptors was probably exerted via different pathways as was indicated by their response to various inhibitors.3