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Anti-SARS-CoV-2 ORF3a Antibody

Anti-SARS-CoV-2 ORF3a Antibody

Item no.	BOS-A33994
Manufacturer	Boster
Amount	100 ug/vial
Category	Antibodies Primary Antibodies
Type	Antibody
Format	Lyophilized
Applications	ELISA
Specific against	Human (Homo sapiens)
Host	Rabbit
Isotype	IgG
Sensitivity	>5000 cells
Citations	1. Gordon DE, et al. Nature, 2020 Jul. 2. Gordon DE, et al. Science, 2020 Oct 15. 3. Hassan SS, et al. Genomics, 2020 Sep. 4. Wu F, et al. Nature, 2020 Mar.
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	ORF3a protein; ORF3a; Accessory protein 3a; Protein 3a; Protein U274; Protein X1
Available
Manufacturer - Category	Primary Antibodies
Manufacturer - Isotype	Rabbit IgG
Storage Conditions	Store at -20Â°C for one year from date of receipt. After reconstitution, at 4Â°C for one month. It can also be aliquotted and stored frozen at -20Â°C for six months. Avoid repeated freeze-thaw cycles.
Observed Molecular Weight	140 kDa, 130 kDa, 110 kDa
Calculated Molecular weight	16693 MW
Clonality	Polyclonal
Application Details	ELISA, 0.001-0.1?g/ml, Human
Manufacturer - Gene Name	3a
Gene Full Name	ORF3a protein
Background	Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ ORF3a encodes a viral accessory protein. Based on its similarity to other coronavirus proteins, ORF3a protein is thought to be a protein with ion channel activity (viroporin) that activates the NLRP3 inflammasome. ORF3a may also play a role in virus replication and pathogenesis.
Immunogen	MDLFMRIFTIGTVTLKQGEIKDATPSDFVRATATIPIQASggggFQSASKIITLKKRWQLALSKGVHFVCNLggggQSINFVRIIMRLWLCWKCRSKNPLLYDANYFLCWHTNCYDYCIPYNSVTSSIVITSGDGTTSPISEHDYQIGGYTEKWESGVKDCVVLHSYFTSDYYQLYSTQLSTDTGVEHVTFFIYNKIVDEPEEHVQIHTIDGSSGVVNPVMEPIYDEPTTTTSVPL
Contents	Each vial contains 4 mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.
Purification	Immunogen affinity purified.
Reconstitution	Add 0.2 ml of distilled water will yield a concentration of 500 ug/ml.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 ?g/ml.
Manufacturer - Research Category	Protein Phosphorylation, Ser/Thr Kinases, Signal Transduction
Protein Function	Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
Subcellular Localization	Cytoplasm.
Description	Boster Bio Anti-SARS-CoV-2 ORF3a Antibody catalog # A33994. Tested in ELISA applications. This antibody reacts with Human.
Tissue Specificity	Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BOS-A33994-datasheet.pdf

BOS-A33994-safety-datasheet.pdf