Manufacturer |
Bioworld Technology, Inc
|
Category |
|
Type |
Antibody Primary |
Specific against |
Human, Mouse |
Applications |
WB, IF, IP, IHC, ICC, CHIP |
Amount |
100ul |
Host |
Rabbit |
Item no. |
BS90981-100 |
Targets |
GLI3;;HNF1A;;TCF3 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Swiss-Prot |
Q01860(Human) P20263(Mouse) |
Application_all |
WB:1:1, 000-1:5, 000 ICC:1:50-1:200 IHC:1:50-1:200 |
Background |
POU5F1 (POU domain, class 5, transcription factor 1), also known as octamer-binding transcription factor-3 (Oct-3, OTF3), octamer-binding transcription factor-4 (Oct-4, Otf-4) and Oct-3/4, modulates embryonic stem (ES) cell populations by influencing lineage commitment. Oct-3/4 sustains stem-cell self-renewal and differentiation pathways. Transcription factors containing the POU homeodomain regulate tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Oct-3/4 is capable of inducing rapid proliferation and tumorigenic properties of ES cells through activation of the UTF1 gene. In humans, two Oct-3/4 isoforms contribute to influencing the undifferentiated phenotype of ES cells. Oct-3/4 pseudogenes localizing to human chromosomes 10 and 8 are reported to be transcribed in certain cancer cell lines and tissues. |
Product |
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
Purification And Purity |
ProA affinity purified |
Storage And Stability |
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles. |
Specificity |
Oct-3/4 polyclonal antibody detects endogenous levels of Oct-3/4 protein. |
BiowMW |
39 kDa |
Note |
For research use only, not for use in diagnostic procedure. |
Immunogen |
recombinant protein |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.