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Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin

Anti-CD4 (GK1.5) In Vivo Antibody - Low Endotoxin

Item no.	ICH1042-100MG
Manufacturer	IchorBio
Amount	100 mg
Quantity options	100 mg 1 mg 25 mg 50 mg 5 mg
Category	Antibodies In Vivo Antibodies Western Blot Blocking
Type	Antibody
Applications	WB, FC, IP, IHC, Blocking
Clone	GK1.5
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Isotype	IgG2b Kappa
Purity	>95% by SDS-PAGE and HPLC
Citations	Cao et al. Injectable hydrogel loaded with lysed OK-432 and doxorubicin for residual liver cancer after incomplete radiofrequency ablation. Journal of Nanobiotechnology
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3
Shipping condition	Cool pack
Available
Specificity	Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) recognizes an epitope on Mouse CD4
Manufacturer - Applications	Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation
Manufacturer - Category	Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype	Rat IgG2b Kappa
Manufacturer - Targets	CD4
Shipping Temperature	Blue ice
Storage Conditions	anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation	Aggregation level ≤ 5%
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution	The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
Application Notes	Blocking: Anti-Mouse CD4 (clone GK1.5) is useful for In vitro and In vivo studies of T-helper cell functions including blocking of ligand binding and T-helper cell activation. Each investigator should determine their own optimal working dilution for specific applications.
Background	CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.
Concentration	≥ 2.0 mg/ml
Endotoxin	≤ 1.0 EU/mg as determined by the LAL method
Manufacturer - Specificity	Anti-CD4 In Vivo Antibody - Low Endotoxin (GK1.5) recognizes an epitope on Mouse CD4
Purification method	This monoclonal antibody was purified using Protein G
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen	Mouse CTL clone V4
Related Antibodies	Anti-CD4 In Vivo Antibody - Ultra Low Endotoxin [GK1.5] (ICH1042UL)
Isotype Control	Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)
Buffer	ICH3001-100ml; ICH3002-100ml; ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 mg

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Delivery expected until 7/31/2025