Comparison

Anti-CD4 (GK1.5) In Vivo Antibody - Ultra-Low Endotoxin European Partner

Item no. ICH1042UL-100MG
Manufacturer IchorBio
Amount 100 mg
Quantity options 100 mg 25 mg 50 mg 5 mg
Category
Type Antibody Monoclonal
Format Liquid
Applications WB, FC, IP, IHC, Blocking
Clone GK1.5
Specific against Mouse (Murine, Mus musculus)
Host Rat
Isotype IgG2b
Purity >98% by SDS-PAGE and HPLC
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Alias T-cell differentiation antigen L3T4,T-cell surface antigen T4/Leu-3
Shipping Condition Cool pack
Available
Manufacturer - Applications
Western Blot, Blocking, Flow Cytometry, IHC (Frozen), Immunoprecipitation
Manufacturer - Category
Ultra-Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Targets
CD4
Shipping Temperature
Blue ice
Storage Conditions
anti-CD4 In Vivo Antibody - Ultra Low Endotoxin (GK1.5) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Aggregation
Aggregation level ≤ 1%
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Antigen Distribution
The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
Application Notes
Blocking: The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation.
Each investigator should determine their own optimal working dilution for specific applications.
Background
CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.
Concentration
≥ 2.0 mg/ml
Endotoxin
≤ 0.5 EU/mg as determined by the LAL method
Manufacturer - Specificity
Anti-CD4 In Vivo Antibody - Ultra Low Endotoxin (GK1.5) recognizes an epitope on Mouse CD4
Purification method
This monoclonal antibody was purified using Protein G
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen
Mouse CTL clone V4
IMPACT Pathogen Test
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham's rat virus
Toolan's H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus
Related Antibodies
Anti-CD4 In Vivo Antibody - Low Endotoxin [GK1.5] (ICH1042)
Isotype Control
Rat IgG2b In Vivo Isotype Control - Ultra Low Endotoxin [1-2] (ICH2243UL)
Buffer

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 mg
Available: In stock
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