« Back
Home /
Anti-Mouse CD4 In Vivo Antibody - Ultra Low Endotoxin (GK1.5)

Anti-Mouse CD4 In Vivo Antibody - Ultra Low Endotoxin (GK1.5)

Manufacturer	IchorBio
Category	Antibodies In Vivo Antibodies Western Blot Blocking
Type	Antibody
Specific against	Mouse
Clone	GK1.5
Isotype	IgG2b Kappa
Applications	WB, FC, IP, IHC, Blocking
Amount	5mg
Host	Rat
Item no.	ICH1042UL-5MG
Targets	CD4
eClass 6.1	32160702
eClass 9.0	32160702
Available
Manufacturers Product Category	Ultra-Low Endotoxin, anti-mouse
Isotype Information	Rat IgG2b Kappa
Purification method	This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution	The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
Background	CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.
Immunogen	Mouse CTL clone V4
Concentration	≥ 2.0 mg/ml
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Endotoxin	≤ 0.5 EU/mg as determined by the LAL method
Aggregation	Aggregation level ≤ 1%
IMPACT Pathogen Test	We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: - Mycoplasma spp - Mycoplasma pulmonis - Pneumonia virus of mice - Kilham's rat virus - Toolan's H1 virus - Rat parvovirus - Lymphocytic choriomeningitis virus - Rat cytomegalovirus - Sendai virus - Rat coronavirus - Sialodacryoadenitis virus - Rat minute virus - Seoul virus - Mouse adenovirus - Reovirus 3 - Rat theilovirus
Storage	anti-CD4 In Vivo Antibody - Ultra Low Endotoxin (GK1.5) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Application Notes	Blocking: The GK1.5 antibody is able to block CD4 mediated cell adhesion and T cell activation. - Each investigator should determine their own optimal working dilution for specific applications.
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 5mg

available

Delivery expected until 6/6/2024