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Anti-Mouse IL-4 In Vivo Antibody - Low Endotoxin (11B11)

Anti-Mouse IL-4 In Vivo Antibody - Low Endotoxin (11B11)

Manufacturer	IchorBio
Category	Antibodies In Vivo Antibodies Cell Biology Cell Culture Cytokines Flow Cytometry ELISA
Type	Antibody
Specific against	Mouse
Clone	11B11
Isotype	IgG1 Kappa
Applications	FC, ELISA
Amount	0.5mg
Host	Rat
Item no.	ICH1087-0.5MG
Targets	IL4
eClass 6.1	32160702
eClass 9.0	32160702
Available
Aggregation	Aggregation level <= 5%
Application Notes	ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.5-2.0 ug/ml. ELISPOT Capture: This Low Endotoxin Functional Formulation antibody has been reported for use as the capture antibody in a sandwich ELISPOT assay. Aseptically coat plates at a concentration of 2-6 ug/ml is recommended. Neutralization: Anti-Mouse IL-4 (clone 11B11) antibody can neutralize the bioactivity of natural or recombinant IL-4. The Low Endotoxin Functional Formulation purified antibody (Endotoxin <0.1 EU/ug, Azide-Free, 0.2 um filtered) is recommended for neutralization of mouse IL-4 bioactivity in vivo. Each investigator should determine their own optimal working dilution for specific applications.
Background	Interleukin-4 (IL-4) is a pleiotropic immune cytokine secreted by activated TH2 cells that inhibits bone resorption. IL-4 is critical for inducing allergic responses. It has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of CD4+ T-cells into Th2 cells. It is also a key regulator in humoral and adaptive immunity. IL-4 is required for the pathogenesis of atopic diseases and immune regulation.
Concentration	1.0 - 5.0 mg/ml
Endotoxin	<0.5 EU/mg as determined by the LAL method
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Immunogen	Purified Recombinant Mouse IL-4 (>98%)
Product Type	Low Endotoxin, anti-mouse
Purification method	This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Storage	anti-IL-4 In Vivo Antibody - Low Endotoxin (11B11) is stable when stored at 2-8C for 1 week. For long term storage aseptically aliquot in working volumes without diluting and store at -80C. Avoid Repeated Freeze Thaw Cycles.
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.5mg

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Delivery expected until 6/20/2024