Anti-Mouse PD-1 (29F.1A12) In Vivo Antibody - Low Endotoxin

Flow Cytometry, Western Blot, Blocking, IHC (Frozen)

PD-1

anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 µg/ml.
Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3, 6, 9 @ 200 ug per animal per injection) then the tumor will regress, and disappear.
Each investigator should determine their own optimal working dilution for specific applications.

1.0 - 5.0 mg/ml

Anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) recognizes an epitope on Mouse PD-1. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Anti-PD-1 In Vivo Antibody - Ultra Low Endotoxin [29F.1A12] (ICH1091UL)

ICH3001-100ml, ICH3002-100ml, ICH3003-100ml