Comparison

Anti-Mouse PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) European Partner

Manufacturer IchorBio
Category
Type Antibody
Specific against Mouse
Clone 29F.1A12
Isotype IgG2a Kappa
Applications WB, FC, Blocking
Amount 1mg
Host Rat
Item no. ICH1091-1MG
Targets CD279
eClass 6.1 32160702
eClass 9.0 32160702
Available
Aggregation
Aggregation level <= 5%
Antigen Distribution
Induced on splenic T and B lymphocytes, thymocytes, and myeloid cells after stimulation.Subset of double negative thymocytes, activated T and B cells
Application Notes
Western Blotting: To detect Mouse PD-1 this antibody can be used at a concentration of 30 ug/ml. Blocking: The 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. NOTE: The response is model dependent. We have seen some tumor lines in mice that grow progressively. If we treat the animal with anti-PD-1 (days 3, 6, 9 200 ug per animal per injection) then the tumor will regress, and disappear. Each investigator should determine their own optimal working dilution for specific applications.
Background
Programmed death-1 (PD-1), also know as CD279 is a 50-55 kD glycoprotein belonging to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Like the clones RMP1-14 and J43 antibodies, the 29F.1A12 antibody has been shown to block the binding of PD-1 to its ligands in vivo.
Concentration
1.0 - 5.0 mg/ml
Endotoxin
<= 1.0 EU/mg as determined by the LAL method
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Immunogen
PD-1 cDNA followed by PD-1-Ig fusion protein
Product Type
Low Endotoxin, anti-mouse
Purification method
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Storage
anti-PD-1 In Vivo Antibody - Low Endotoxin (29F.1A12) is stable when stored at 2-8C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at -80C. Avoid Repeated Freeze Thaw Cycles.
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1mg
Available: In stock
available

Delivery expected until 6/20/2024 

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