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Anti-Mouse Ly-6G/Ly-6C (RB6-8C5) In Vivo Antibody - Ultra-Low Endotoxin

Anti-Mouse Ly-6G/Ly-6C (RB6-8C5) In Vivo Antibody - Ultra-Low Endotoxin

Item no.	ICH1131UL-50MG
Manufacturer	IchorBio
Amount	50 mg
Quantity options	100 mg 25 mg 50 mg 5 mg
Category	In Vivo Antibodies In Vivo Antibodies Flow Cytometry Western Blot
Type	Antibody
Applications	WB, FC, IF, IP, IHC, ICC, FA
Clone	RB6-8C5
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Isotype	IgG2b
Purity	>98% by SDS-PAGE and HPLC
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Ly-6G/LY-6C, Gr-1, Myeloid
Shipping condition	Cool pack
Available
Manufacturer - Applications	Immunoprecipitation, Western Blot, Flow Cytometry, Functional Assays, IHC (Frozen), IHC (Paraffin), Immunocytochemistry, Immunofluorescence, In Situ Hybridization
Manufacturer - Category	Ultra-Low Endotoxin, anti-mouse, monoclonal antibody
Manufacturer - Isotype	Rat IgG2b
Manufacturer - Targets	Ly-6G/Ly-6C
Shipping Temperature	Blue ice
Storage Conditions	anti-Ly-6G/Ly-6C In Vivo Antibody - Ultra Low Endotoxin (RB6-8C5) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Aggregation	Aggregation level ≤ 1%
Formulation	No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.
Antigen Distribution	The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells.
Application Notes	Each investigator should determine their own optimal working dilution for specific applications.
Background	Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C.
Concentration	0.5 mg/ml
Endotoxin	≤ 0.5 EU/mg as determined by the LAL method
Manufacturer - Specificity	Rat Anti-Mouse Granulocytes (Clone RB6-8C5) recognizes Mouse Granulocytes
Purification method	This monoclonal antibody was purified using Protein G
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunogen	Raised against granulocytes of mouse origin
IMPACT Pathogen Test	We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for: Mycoplasma spp Mycoplasma pulmonis Pneumonia virus of mice Kilham's rat virus Toolan's H1 virus Rat parvovirus Lymphocytic choriomeningitis virus Rat cytomegalovirus Sendai virus Rat coronavirus Sialodacryoadenitis virus Rat minute virus Seoul virus Mouse adenovirus Reovirus 3 Rat theilovirus
Related Antibodies	Anti-Ly-6G/Ly-6C In Vivo Antibody - Low Endotoxin [RB6-8C5] (ICH1131)
Antibodies against the same target (Cat No.)	ICH1111-1MG, ICH1095-1MG, ICH1111-1MG, ICH1089-1MG
Isotype Control	Rat IgG2b In Vivo Isotype Control - Ultra Low Endotoxin [1-2] (ICH2243UL)
Buffer	ICH3001-100ml; ICH3002-100ml; ICH3003-100ml

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50 mg

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Delivery expected until 6/5/2025