Manufacturer |
IchorBio
|
Category |
|
Type |
Antibody |
Specific against |
Mouse |
Clone |
RMP1-14 |
Isotype |
IgG2a Kappa |
Applications |
WB, FC, Blocking, FA |
Amount |
100mg |
Host |
Rat |
Item no. |
ICH1132-100MG |
Targets |
CD279 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Manufacturers Product Category |
Low Endotoxin, anti-mouse |
Target Information |
PD-1 |
Isotype Information |
Rat IgG2a Kappa |
Purification method |
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. |
Antigen Distribution |
Subset of double negative thymocytes, activated T and B cells |
Background |
CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. This RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production. |
Immunogen |
Mouse PD-1 transfected BHK cells |
Concentration |
1.0 - 5.0 mg/ml |
Formulation |
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
Endotoxin |
≤ 1.0 EU/mg as determined by the LAL method |
Aggregation |
Aggregation level ≤ 5% |
Storage |
anti-PD-1 In Vivo Antibody - Low Endotoxin (RMP1-14) is stable when stored at 2-8°C. For longer term storage (> 3 months) it is recommended to store this antibody at -70oC. |
Application Notes |
Blocking: Clone RMP1-14 antibody has been shown to block the binding of both mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1. For immune checkpoint antibodies 200 ug per mouse per injection is the standard dosage in the literature. For this anti-PD-1 (clone RMP1-14), we suggest to inject 200 ug/mouse ip generally on days 3, 6 and 9, post injection of tumor. Sometime up to six injections depending on the length of the experiment may be required. - Each investigator should determine their own optimal working dilution for specific applications. |
Use |
Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.